【摘 要】
：
Erectile dysfunction and penile shrinkage are the common complications after radical prostatectomy.Penile rehabilitation is widely applied after the surgery.Vacuum therapy is one of the three penile rehabilitation methods used in the clinical setting,but
【机 构】
：
Andrology Laboratory, West China Hospital, Sichuan University, Chengdu 610041, China;Department of U
论文部分内容阅读
Erectile dysfunction and penile shrinkage are the common complications after radical prostatectomy.Penile rehabilitation is widely applied after the surgery.Vacuum therapy is one of the three penile rehabilitation methods used in the clinical setting,but its mechanism is not well known.This study was designed to investigate whether vacuum erectile device (VED) can prevent corporeal veno-occlusive dysfunction and penile shrinkage in the bilateral cavernous nerve crush (BCNC) rat model.Adult male Sprague-Dawley rats were randomly assigned into three groups:sham group,BCNC group,and BCNC + VED group.After 4 weeks,penile length and intracavernosal pressure (ICP) were measured,and then the middle part of the penis was harvested after dynamic infusion cavernosometry to complete the following items:smooth muscle/collagen ratios and collagen Ⅰ/Ⅲ ratios;ultramicrostructure of the tunica albuginea,endothelial cell,and smooth muscle cell;and the expression of calponin-1 and osteopontin.The penile shortening,peak ICP and ICP drop rate after alprostadil injection were significantly improved with vacuum therapy after 4-week treatment.Compared with BCNC group,VED significantly increased smooth muscle/collagen ratios,decreased collagen Ⅰ/Ⅲ ratios,and preserved the ultramicrostructure of the tunica albuginea,endothelial cell,and smooth muscle cell.The data also showed that animals exposed to VED could partially reverse the expression of calponin-1 and osteopontin induced by BCNC.In conclusion,vacuum therapy is effective to prevent penile shrinkage and veno-occlusive dysfunction in penile rehabilitation,which may be associated with well-preserved structure and function of the tunica albuginea,endothelial cell,and smooth muscle cell.
其他文献
We summarized our experience in transurethral seminal vesiculoscopy (TSV) for recurrent hemospermia by introducing surgical techniques, intraoperative findings, and treatment outcomes. TSV was performed in 419 patients with an initial diagnosis of persist
During spermatogenesis,developing germ cells that lack the cellular ultrastructures of filopodia and lamellipodia generally found in migrating cells,such as macrophages and fibroblasts,rely on Sertoli cells to support their transport across the seminifero
Human papillomavirus (HPV) is the most common sexually transmitted virus in males and females worldwide; yet its impact upon male fertility remains unclear. The objective of this study was to evaluate the potential impact of HPV infection in semen on male
Cryptorchidism is associated with infertility in adulthood.Early orchiopexy is suggested to reduce the risk.Information is lacking on the potential link between infant germ cell maturation and the risk of future infertility.The objective of the study was
Prostate cancer (PCa) exhibits epidemiological and molecular heterogeneity.Despite extensive studies of its phenotypic and genetic properties in Western populations,its molecular basis is not clear in Chinese patients.To determine critical molecular chara
This study compared the diagnostic efficacy of transrectal ultrasound (TRUS)-guided prostate biopsy (TRBx) and transperineal prostate biopsy (TPBx) in patients with suspected prostate cancer (PCa).We enrolled 2962 men who underwent transrectal (n =1216) o
Male germ cells are the only adult cells to undergo the intricate process of meiosis,which is preceded by the complex molecular transition from mitotic spermatogonia to spermatocytes.Recent single-cell transcriptomic studies have shown that this transitio
This study aimed to further validate the prognostic role of fibrinogen in upper tract urothelial carcinoma (UTUC) in a large Chinese cohort.A total of 703 patients who underwent radical nephroureterectomy were retrospectively identified.Fibrinogen levels
Spermatogonial stem cells (SSCs) transmit genetic information to the next progeny in males.Thus,SSCs are a potential target for germline modifications to generate transgenic animals.In this study,we report a technique for the generation of transgenic rats
We aimed to develop and validate a clinical nomogram predicting bladder outlet obstruction (BOO) solely using routine clinical parameters in men with refractory nonneurogenic lower urinary tract symptoms (LUTS).A total of 750 eligible patients ＞50 years o