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目的生物监测法,是根据其微生物的死亡情况以判断灭菌是否成功.它是确定压力灭菌物品达到灭菌的可靠方法。如果生物监测培养结果阳性,那么.灭菌质量就得不到保证.影响生物监测培养结果的因素有生物指示剂有效性.规范的操作技术及灭菌器的性能外,还必须保证培养锅的性能完好.避免生物监测结果的假阳性.方法利用嗜热脂肪杆菌芽胞的活菌放于标准测试包中心,经过一个灭菌过程后,对灭菌效果进行监测.结果连续三次生物监测,检验科3次培养结果阳性.同时送外院培养2次全部阴性.结论培养锅不恒温引起.
The purpose of the biological monitoring method is to determine whether the sterilization is successful or not based on the death of the microorganism and it is a reliable method of determining that the sterilized items are sterilized. If the biomonitoring results are positive, then the quality of the sterilization can not be guaranteed.The factors influencing the biological monitoring of the culture results are the effectiveness of the biological indicator, the standard operating techniques and the performance of the sterilizer, The performance is good.Avoid the false positive of biological monitoring results.METHODS The viable bacteria of Bacillus stearothermophilus were placed in the center of standard test package and the sterilization effect was monitored after a sterilization process.Results Three consecutive biological monitoring and laboratory tests 3 times the culture results were positive.At the same time to send outside the hospital culture all 2 negative.Conclusion The incubation pot is not constant temperature caused.