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目的测定不同产地及不同采收季节的积雪草中积雪草苷、羟基积雪草苷的含量。方法采用Agilent C18色谱柱,流动相为乙腈-水,梯度洗脱,流速1.0 m.lmin-1,检测波长205 nm,柱温30℃。结果积雪草苷和羟基积雪草苷平均的回收率分别为99.5%、100.3%,RSD分别为1.06%、1.21%(n=6)。结论不同产地中的积雪草苷、羟基积雪草苷的含量有差异。广西南宁地区产积雪草中,6~10月采收的积雪草中积雪草苷、羟基积雪草苷含量较高。
Objective To determine the content of asiaticoside and madecassoside in Centella asiatica from different producing areas and different harvesting seasons. Methods The Agilent C18 column was used. The mobile phase consisted of acetonitrile-water with gradient elution at a flow rate of 1.0 m.lmin-1. The detection wavelength was 205 nm and the column temperature was 30 ℃. Results The average recoveries of asiaticoside and madecassoside were 99.5% and 100.3%, respectively. The RSDs were 1.06% and 1.21%, respectively (n = 6). Conclusion There are differences in the contents of asiaticoside and madecassoside in different habitats. Among the products of Centella asiatica in Nanning, Guangxi, Centella asiatica collected from June to October had a high content of Madecassoside.