论文部分内容阅读
目的研究山东省13个哨点县2004—2007年非结核分枝杆菌临床分离率和菌种分布情况。方法对山东省13个哨点县2004—2007年送到汉光中心的2 625株分枝杆菌菌株,进行分枝杆菌菌群鉴定试验,鉴定为非结核分枝杆菌的菌株应用16SrDNA测序方法进行菌种鉴定。结果2 625株菌株分枝杆菌菌群鉴定39株为非结核分枝杆菌菌群,39株菌株16SrDNA序列分析结果有36株是非结核分枝杆菌,其中29株为胞内分枝杆菌株(80.6%),其余分别为堪萨斯分枝杆菌、偶然分枝杆菌各2株、戈登分枝杆菌、龟脓肿分枝杆菌复合物、瘰疬分枝杆菌各1株;结核分枝杆菌复合群2株;鼻疽诺卡氏菌1株。山东地区非结核分枝杆菌临床分离率占分枝杆菌培养阳性菌株的1.4%。结论山东地区流行的非结核分枝杆菌以慢生长分枝杆菌的胞内分枝杆菌为主。
Objective To study the clinical isolates and distribution of non-tuberculous mycobacteria in 13 sentinel counties in Shandong Province during 2004-2007. Methods Twenty-six strains of Mycobacterium that were sent to Hankuang Center from 13 sentinel counties in Shandong Province in 2004-2007 were tested for mycobacterium flora. The strains identified as non-tuberculous mycobacteria were sequenced by 16S rDNA sequencing Identification of bacteria. Results The results showed that 39 strains of Mycobacterium tuberculosis were identified as Mycobacterium tuberculosis in 2625 isolates and 36 strains of non-Mycobacterium were found in 16 isolates of 39 strains. Among them, 29 strains were Mycobacterium intracellulare (80.6 %), The rest were Kansas M. marcescens, two strains of Mycobacterium fortuitum each, Mycobacterium Gordon, Mycobacterium turtle abscess complex, Mycobacterium bovis 1 strain each; Mycobacterium tuberculosis complex 2 ; Nocardia Nocardia 1 strain. The clinical isolation rate of Mycobacterium tuberculosis in Shandong accounted for 1.4% of the positive strains of Mycobacterium culture. Conclusion The prevalence of Mycobacterium tuberculosis in Shandong is mainly Mycobacterium intracellulare with slow-growing Mycobacterium.