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目的:研究三棱丸(Sparganii Pill SP)对转化生长因子β1(TGF-β1)诱导的间质化肠上皮细胞(IEC-6)钙黏蛋白E(E-cadherin)、α-平滑肌肌动蛋白(α-smooth muscle actinα-SMA)及过氧化物酶体增殖物激活受体γ(PPARγ)表达的影响及其对IEC-6细胞TGFβ1/Smads信号转导途径的影响及可能机制。方法:以IEC-6细胞为研究对象,用Western blot法检测高、中、低剂量的SP及罗格列酮含药血清作用后的PPARγ、Smad2/3及p-Smad3蛋白的表达,RT-PCR检测E-cadherin、α-SMA mRNA的表达,观察SP及罗格列酮对PPARγ、pSmad3入核水平的影响。结果:与10%正常大鼠血清组比较,SP含药血清组能够抑制α-SMA mRNA表达水平;提高E-cadherin、PPARγ,降低pSmad3、α-SMA的蛋白表达,尤以20%SP含药血清组作用明显;10%SP含药血清组促进PPARγ入核,抑制pSmad3的入核。结论:在体外,SP抑制TGFβ1/Smads信号通路途径转导IEC-6细胞EMT的机制可能与激活PPARγ的表达有关。
OBJECTIVE: To investigate the effect of Sparganii Pill SP on the expression of E-cadherin, α-smooth muscle actin (IL-1β) in interstitial intestinal epithelial cells induced by transforming growth factor β1 (TGF- (SMA) and peroxisome proliferator activated receptor γ (PPARγ) in IEC-6 cells and its possible mechanism. Methods: The expression of PPARγ, Smad2 / 3 and p-Smad3 protein in IEC-6 cells were detected by Western blot assay. The expression of PPARγ, Smad2 / 3 and p- PCR was used to detect the expression of E-cadherin and α-SMA mRNA. The effects of SP and rosiglitazone on the nuclear import of PPARγ and pSmad3 were observed. Results: Compared with 10% normal rat serum group, SP-containing serum group could inhibit the expression of α-SMA mRNA, enhance the expression of E-cadherin and PPARγ and decrease the expression of pSmad3 and α-SMA, especially 20% SP Serum group has obvious effect; 10% SP drug-containing serum group can promote PPARγ into nucleus and inhibit pSmad3 into nucleus. Conclusion: In vitro, the mechanism by which SP inhibits TGFβ1 / Smads signaling pathways transducing EMT in IEC-6 cells may be related to the activation of PPARγ expression.