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三七,又名田七,性温、味微苦,既能活血化瘀,又擅止血定痛,是临床防治肝病常用的一种中药材。三七总甙(Panax notoginseng saponins,简称PNS,下同)是三七中的一类主要化学成分,研究表明,有改善微循环、保护肝细胞、促进肝细胞再生等功能。肝纤维化是肝细胞损伤后所引起的大量细胞外基质在肝内增生沉积的一种病理改变。慢性肝病有此特征性病理变化。为了探讨PNS是否具有抗肝纤维化活性,本文观察了PNS及三七对成纤维细胞增殖及Ⅰ型胶原合成与分泌的影响。1 材料1.1 NIH/~3T3细胞(小鼠胚胎成纤维细胞)株 购自中
Sanqi, also known as Tian Qi, is a traditional Chinese herbal medicine used for clinical prevention and treatment of liver diseases. It has a mild temperature and a bitter bitter taste. It can not only promote blood circulation, but also stop bleeding. Panax notoginseng saponins (PNS, the same below) is a major chemical component of Panax notoginseng. Studies have shown that it can improve microcirculation, protect liver cells, and promote hepatocyte regeneration. Liver fibrosis is a pathological change in the accumulation of a large number of extracellular matrices in the liver caused by liver cell injury. Chronic liver disease has this characteristic pathological change. To investigate whether PNS has anti-hepatic fibrosis activity, the effects of PNS and Panax notoginseng on proliferation of fibroblasts and synthesis and secretion of type I collagen were observed. 1 Materials 1.1 NIH/~3T3 cells (mouse embryonic fibroblast) strains purchased from