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通过问卷调查为主要方式,个案追踪调查等方式为辅,对昆明卫生职业学院12级和13级高职护理班学生的生物化学学习情况进行调查与分析,探讨高职高专学生学习生物化学困难的主要原因,帮助教师明晰生化教学对象面临的问题,为今后的教学提出对策,以提高教师教学质量,提高学生生化学习效果。“,”This paper conducted a questionnaire survey to get to know students' cur ent situation of learning biochemistry,the object of study came from Nursing students of grade 12 and 13 in the kunming health professional col ege. And then the paper analyzes causes for dif iculties in learning the course.Improving the teaching ef ect of this course such as reforming teaching contents,and improving students' learning af ect.