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目的:前期的研究发现Stab1为GBM的一个特异性差异表达基因,本文通过对其他GBM表达公共数据库的进一步挖掘结合实验验证从而推测STAB1的表达与GBM生存之间的关系。方法:对TCGA数据库中的GBM基因表达谱芯片数据进行Cox回归分析和生存分析;用免疫组织化学实验方法检测湖南省第二人民医院病理科1999—2012年间GBM肿瘤31例中STAB1的表达情况,并分析其与GBM生存之间的关系。结果:用Cox回归分析和生存分析以及结合免疫组化实验发现STAB1的表达与GBM的生存有显著相关性(HR=1.96,P=1.98e-04),为GBM预后的一个危险因素。在GBM病人中,STAB1阳性表达组生存时间显著低于阴性表达组。结论:结合STAB1潜在促进血管生成的功能,本文从临床关联的角度进一步说明STAB1在GBM中的重要性。
OBJECTIVE: The previous study found that Stab1 is a specific differentially expressed gene of GBM. In this paper, we further speculate that the relationship between the expression of STAB1 and the survival of GBM through the further mining of public databases of other GBM expression combined with experimental validation. Methods: Cox regression analysis and survival analysis of GBM gene expression microarray data in TCGA database were performed. The expression of STAB1 in 31 cases of GBM tumor from 1999 to 2012 in Department of Pathology, Second People’s Hospital of Hunan Province was detected by immunohistochemistry. And analyze its relationship with GBM survival. Results: Cox regression analysis and survival analysis combined with immunohistochemistry showed that there was a significant correlation between the expression of STAB1 and the survival of GBM (HR = 1.96, P = 1.98e-04), which was a risk factor for the prognosis of GBM. In GBM patients, STAB1-positive expression group survival time was significantly lower than the negative expression group. Conclusions: Combined with the potential role of STAB1 in promoting angiogenesis, this article further illustrates the importance of STAB1 in GBM from a clinical perspective.