论文部分内容阅读
伤寒沙门菌和副伤寒沙门菌是引起肠热症的病因,在发展中国家是威胁人们健康的重要问题。两者临床症状难于区分,确切的病原学检测及鉴别诊断非常必要。本文回顾了伤寒从分离培养、血清学方法到PCR方法,以及鉴别伤寒和甲型副伤寒沙门菌感染的几种多重PCR方法。这几种多重PCR方法分别以扩增rfb基因簇中伤寒和甲型副伤寒沙门菌各自特异基因,扩增O抗原、Vi抗原、鞭毛蛋白编码基因及扩增多个特异的蛋白编码序列达到鉴别诊断的目的。
Salmonella typhi and Salmonella paratyphi are the causes of enteric fever, which is an important issue threatening people’s health in developing countries. Difficult to distinguish between the clinical symptoms of the two, the exact pathogen detection and differential diagnosis is necessary. This article reviews several multiplex PCR methods for the isolation and culture of typhoid fever, serological methods, and PCR methods, as well as for the identification of typhoid and paratyphoid Salmonella paratyphi A infections. These multiplex PCR methods were respectively used to amplify the specific genes of typhi and typhi Salmonella typhi in the rfb gene cluster and amplify O antigen, Vi antigen, flagellin coding gene and amplify multiple specific protein coding sequences respectively The purpose of diagnosis.