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目的:探讨血清乙二醛酶I在2型糖尿病眼肌麻痹(DOMP)中的意义及其与晚期蛋白氧化产物(AOPP)及氧化应激的相关性。方法:分析58例DOMP、50例单纯T2DM及30例正常受试者的临床资料,测定受试者血脂、空腹血糖、糖化血红蛋白、胰岛素、乙二醛酶I、AOPP及氧化应激指标包括丙二醛(MDA)、超氧化物歧化酶(SOD)、总抗氧化力(T-AOC),采用稳态模型计算胰岛素抵抗指数。结果:高密度脂蛋白、SOD、T-AOC与乙二醛酶I呈正相关而与AOPP呈负相关,甘油三酯、低密度脂蛋白、空腹血糖、糖化血红蛋白、胰岛素抵抗指数、MDA与乙二醛酶I呈负相关而与AOPP呈正相关。AOPP与乙二醛酶I高度负相关(r=-0.823,P<0.001)。多元线性回归分析提示课题分组是乙二醛酶、AOPP的重要影响因素,即按DOMP、单纯T2DM及正常受试者的分组顺序乙二醛酶逐级升高(Sβ=0.554)而AOPP逐级下降(Sβ=-0.469)。结论 :DOMP血清乙二醛酶I显著降低,并与AOPP及氧化应激密切相关,提示乙二醛酶I可能在抑制DOMP发生发展中有重要作用。
Objective: To investigate the significance of serum glyoxalase I in ophthalmoplegia (DOMP) of type 2 diabetes mellitus (DM) and its relationship with advanced protein oxidation products (AOPP) and oxidative stress. Methods: The clinical data of 58 cases of DOMP, 50 cases of simple T2DM and 30 normal subjects were analyzed. The levels of serum lipids, fasting blood glucose, glycosylated hemoglobin, insulin, glyoxalse I, AOPP and oxidative stress indexes including C (MDA), superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) were calculated. The insulin resistance index was calculated by steady-state model. Results: There was a positive correlation between HDL, SOD, T-AOC and glyoxalase I and negatively correlated with AOPP. The levels of triglyceride, LDL, fasting blood glucose, glycosylated hemoglobin, insulin resistance index, Aldolase I was negatively correlated with AOPP was positively correlated. AOPP was highly negatively correlated with glyoxalase I (r = -0.823, P <0.001). Multivariate linear regression analysis suggested that the subgroups of glyoxalase and AOPP were the main influencing factors of glyoxalase and AOPP. The glyoxalase increased gradually (Sβ = 0.554) and the level of AOPP gradually increased according to the grouping order of DOMP, simple T2DM and normal subjects Decreased (Sβ = -0.469). CONCLUSION: The level of glyoxalase I in serum of the patients with DOMP is significantly decreased, which is closely related to AOPP and oxidative stress, suggesting that glyoxalase I may play an important role in inhibiting the occurrence and development of DOMP.