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目的探讨骨桥蛋白及基质金属蛋白酶-9(MMP-9)在人恶性黑素瘤中的表达及意义。方法采用免疫组化SP法检测23例原发性恶性黑素瘤、17例转移性恶性黑素瘤及20例色素痣中骨桥蛋白和MMP-9的表达。结果40例恶性黑素瘤中骨桥蛋白及MMP-9表达的阳性率分别为87．5％、75．0％；20例色素痣中的阳性表达率分别为15．0％、10．0％。骨桥蛋白及MMP-9在恶性黑素瘤中阳性表达率明显高于色素痣,差异具有统计学意义(P＜0．05)。骨桥蛋白和MMP-9的表达与年龄、性别、发病部位、是否淋巴结转移等因素均无关(P＞0．05)。40例恶性黑素瘤中,骨桥蛋白与MMP-9均表达29例,均不表达4例。结论骨桥蛋白及MMP-9在人恶性黑素瘤中高表达,但与淋巴结转移无相关性。
Objective To investigate the expression of osteopontin and matrix metalloproteinase-9 (MMP-9) in human malignant melanoma and its significance. Methods The expression of osteopontin and MMP-9 in 23 cases of primary malignant melanoma, 17 cases of metastatic malignant melanoma and 20 cases of pigmented nevus were detected by immunohistochemical SP method. Results The positive rates of osteopontin and MMP-9 in 40 cases of malignant melanoma were 87.5% and 75.0%, respectively. The positive expression rates of osteopontin and MMP-9 in 20 cases were 15.0% and 10.0% respectively %. The positive rate of osteopontin and MMP-9 in malignant melanoma was significantly higher than that of pigmented nevus, the difference was statistically significant (P <0.05). The expression of osteopontin and MMP-9 had no correlation with age, sex, site of disease and lymph node metastasis (P> 0.05). In 40 cases of malignant melanoma, osteopontin and MMP-9 were expressed in 29 cases, none of which were expressed in 4 cases. Conclusion Osteopontin and MMP-9 are highly expressed in human malignant melanoma but not in lymph node metastasis.