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目的比较灵芝及灵芝提取物和相关产品中多糖含量测定2种方法的差异。方法采用苯酚-硫酸法,蒽酮-硫酸法比色测定灵芝多糖含量。结果该2种方法测定实验结果基本一致,标准葡聚糖液在0.005～0.1 mg/ml浓度范围内线性关系良好,相关系数均大于0.999 4。结论该2种方法测定结果准确可靠,可用于灵芝多糖的含量测定。
Objective To compare the differences of two methods in the determination of polysaccharide in Ganoderma lucidum and Ganoderma lucidum extracts and related products. Methods The contents of Ganoderma lucidum polysaccharides were determined by phenol - sulfuric acid method and anthrone - sulfuric acid method. Results The results of the two methods were basically the same. The linearity of standard dextran solution was good in the concentration range of 0.005-0.1 mg / ml, and the correlation coefficient was higher than 0.999 4. Conclusion The results of the two methods are accurate and reliable and can be used for the determination of Ganoderma lucidum polysaccharides.