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目的:对瓜蒌薤白滴丸及其组方药味的抗心肌缺血药效进行研究。方法:将SPF级SD大鼠随机分为11组,每组10只,雌雄各半,正常对照组与模型组灌胃生理盐水,药物组9组,分别灌胃给予瓜蒌、薤白及瓜蒌薤白白酒汤(均相当于生药22.5 g/kg),瓜蒌薤白滴丸3.75、11.25、22.5、33.75、45 g/kg及复方丹参滴丸0.085 g/kg,1次/d,干预7 d后,除正常对照组外,其余各组腹腔注射ISO制备大鼠急性心肌缺血模型,观察各组大鼠心电图ST段的变化;测定血浆SOD、NO、HDL-C、MDA、CAT、LDH、CK水平;对心肌组织进行HE染色,观察组织病理变化。结果:各给药组与模型组比较,瓜蒌薤白滴丸22.5、33.75、45 g/kg组分别有10、11、12个时间点ST段明显回落(P<0.05),回落时间点数多于其他各组;瓜蒌薤白滴丸22.5、33.75 g/kg能同时显著改善模型大鼠血浆SOD、MDA、CAT、NO、HDL-C、LDH及CK水平(P<0.05或P<0.01);瓜蒌薤白滴丸各剂量均能改善模型大鼠心肌组织病理学改变。结论:瓜蒌薤白滴丸能有效对抗ISO所致的大鼠急性心肌缺血,效果优于瓜蒌、薤白单用及瓜蒌薤白白酒汤。
OBJECTIVE: To study the anti-myocardial ischemia pharmacological effects of Guluo Baitiao Wan and its proprietary prescriptions. Methods: SPF SD rats were randomly divided into 11 groups, 10 in each group, male and female in half. Normal control group and model group were given normal saline and drug group, and were given gavage,薤 white wine soup (equivalent to 22.5 g / kg crude drug), melon 蒌 Baishidao pills 3.75,11.25,22.5,33.75,45 g / kg and compound Danshen dripping pills 0.085 g / kg, 1 / d, after intervention for 7 d , Except for the normal control group, the other groups were intraperitoneally injected with ISO to prepare rat model of acute myocardial ischemia, ECG changes of ST segment in each group were observed. Plasma SOD, NO, HDL-C, MDA, CAT, LDH and CK Level; myocardial tissue HE staining, histopathological changes observed. Results: Compared with the model group, the ST segment of Guantixiao Baidiao Wan in 22.5,33.75 and 45 g / kg groups were significantly decreased at 10, 11 and 12 points (P <0.05), and the number of fall points was more than (P <0.05 or P <0.01). The levels of SOD, MDA, CAT, NO, HDL-C, LDH and CK in plasma of model group were also improved significantly Each dose of Baisha Baidiao Wan can improve the myocardial histopathological changes in model rats. Conclusion: Guadiaobai dripping pills can effectively prevent acute myocardial ischemia induced by ISO in rats.