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目的观察茯苓、甘草、银花复方制剂对砷中毒小鼠治疗效果,寻找一种安全有效无毒的治疗砷中毒药物。方法建立砷中毒小鼠动物模型,按随机方法将小鼠分为对照组、砷中毒组和治疗组;治疗组先行染砷再给予茯苓、甘草、银花复方制剂进行治疗;各组处理60d后测定血清中超氧化物歧化酶(SOD)活力、谷胱甘肽(GSH)和肌酐(Cr)含量;行肝脏、肾脏组织切片,光镜观察肝、肾损害程度。结果砷中毒组小鼠SOD活力下降[(129.86±37.23)UN/ml],GSH含量降低[(172.16±30.08)mg/L],Cr含量升高[(123.56±23.69)μmol/L],小鼠肝、肾脏损害严重;治疗组SOD活力、GSH和Cr含量明显恢复,分别是(189.68±47.16)UN/ml、(266.29±58.45)mg/L、(86.75±16.08)μmol/L,肝脏、肾脏损害明显减轻。结论茯苓、甘草、银花复方制剂对染砷中毒小鼠具有明显的治疗效果。
Objective To observe the therapeutic effect of Poria, licorice, Yin-hua compound on arsenic poisoning mice and to find a safe, effective and nontoxic treatment of arsenic poisoning drugs. Methods The animal model of arsenic poisoning mice was established. The mice were randomly divided into control group, arsenic poisoning group and treatment group. The treatment group was treated with arsenic and then with Poria, licorice and Yinhua compound. After 60 days of treatment, Serum superoxide dismutase (SOD) activity, glutathione (GSH) and creatinine (Cr) contents were measured. The liver and kidney sections were stained with light microscope to observe the degree of liver and kidney damage. Results The SOD activity in arsenism mice was decreased (129.86 ± 37.23) U / ml, the content of GSH was decreased (172.16 ± 30.08 mg / L) and the content of Cr was increased (123.56 ± 23.69 μmol / L) The liver, kidney and liver of rats in the treatment group were severely damaged. The activity of SOD and the content of GSH and Cr in the treatment group were significantly recovered (189.68 ± 47.16) / ml, (266.29 ± 58.45) mg / L and 86.75 ± 16.08 μmol / L, Kidney damage significantly reduced. Conclusion Poria, licorice, Yin-hua compound preparations of arsenic poisoning in mice has a significant therapeutic effect.