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目的探讨甘草酸(GA)对实验性哮喘小鼠气道炎症的影响及其可能机制。方法 40只BALB/c小鼠采用随机数字表法分为对照组、哮喘组和低、中、高浓度GA干预组,每组8只。哮喘组和GA干预组小鼠分别给予卵清白蛋白(OVA)致敏和激发。每次雾化前30min,干预组分别给予GA(25、50、100mg/kg)腹腔注射。末次激发后,摘眼球取血,收集肺泡灌洗液(BALF),离心后计数细胞总数及白细胞分类。ELISA法测定血浆免疫球蛋白E(IgE)和BALF上清液中IL-4和IL-5的水平。肺组织免疫组化染色,Image Pro Plus图像分析系统分析Eotaxin、MCP-1和CINC-1的平均吸光度值。结果哮喘组BALF中细胞总数及嗜酸粒细胞、淋巴细胞和中性粒细胞计数均大于对照组(P<0.05)。中、高浓度GA干预组BALF中细胞总数及嗜酸粒细胞和淋巴细胞计数均少于哮喘组(P<0.05)。哮喘组小鼠血浆IgE和BALF中IL-4、IL-5的水平高于对照组(P<0.05);GA干预组血浆IgE和BALF中IL-4、IL-5水平低于哮喘组;且随着GA干预浓度的增加,此种作用愈加明显(P<0.05)。哮喘组、GA干预组小鼠Eotaxin、MCP-1和CINC-1蛋白的表达均高于对照组(P<0.05),GA干预组小鼠Eotaxin、MCP-1和CINC-1蛋白的表达均低于哮喘组(P<0.05)。结论 GA可能通过下调趋化因子Eotaxin、MCP-1和CINC-1的表达,从而发挥减轻气道炎症的作用。
Objective To investigate the effect of glycyrrhizin (GA) on airway inflammation in experimental asthmatic mice and its possible mechanism. Methods 40 BALB / c mice were randomly divided into control group, asthma group and low, medium and high concentration GA intervention groups, with 8 in each group. The asthma group and GA intervention group were given ovalbumin (OVA) sensitized and challenged respectively. At 30 minutes before the nebulization, the intervention group was given intraperitoneal injection of GA (25, 50 and 100 mg / kg) respectively. After the last challenge, blood was taken from the eye and BALF was collected and the total number of cells and leukocyte count were counted after centrifugation. The levels of IL-4 and IL-5 in plasma immunoglobulin E (IgE) and BALF supernatant were measured by ELISA. Immunohistochemical staining of lung tissue and average absorbance values of Eotaxin, MCP-1 and CINC-1 were analyzed by Image Pro Plus image analysis system. Results The total number of BALF cells, the number of eosinophils, lymphocytes and neutrophils in asthma group were significantly higher than those in control group (P <0.05). The total number of BALF and the number of eosinophils and lymphocytes in the medium and high concentration GA intervention groups were less than those in the asthma group (P <0.05). The level of IL-4 and IL-5 in plasma IgE and BALF in asthmatic mice was higher than that in control group (P <0.05). The level of IL-4 and IL-5 in plasma IgE and BALF in GA-treated group was lower than that in asthma group With the GA intervention concentration increased, the effect of the more obvious (P <0.05). The expressions of Eotaxin, MCP-1 and CINC-1 in asthma group and GA intervention group were significantly higher than those in control group (P <0.05). The expression of Eotaxin, MCP-1 and CINC- In the asthma group (P <0.05). Conclusion GA may play an important role in reducing airway inflammation by down-regulating the expression of chemotactic factors Eotaxin, MCP-1 and CINC-1.