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目的检测ORF3融合蛋白对L02细胞增殖与凋亡的影响。方法将重组质粒ORF3-pEGFP和ORF3-pXF2RH脂质体法转染L02细胞,用MTT比色法测定细胞增殖情况,用流式细胞仪碘化丙啶染色法检测细胞周期和细胞凋亡情况。结果转染重组质粒的L02细胞自第4d开始,明显增殖(P<0.05);L02细胞凋亡率由转染前的1.41%分别下降为0.83%和0.88%,差异有统计学意义(P<0.05)。结论戊型肝炎ORF3融合蛋白可减少L02细胞凋亡,而使L02增殖速度增加(P<0.05)。
Objective To detect the effect of ORF3 fusion protein on the proliferation and apoptosis of L02 cells. Methods L02 cells were transfected with recombinant plasmids ORF3-pEGFP and ORF3-pXF2RH by liposome method. Cell proliferation was measured by MTT assay. Cell cycle and apoptosis were detected by flow cytometry with propidium iodide staining. Results The L02 cells transfected with the recombinant plasmids showed significant proliferation (P <0.05), and the apoptosis rate of L02 cells decreased from 1.41% before transfection to 0.83% and 0.88%, respectively, with significant difference (P < 0.05). Conclusion HEV ORF3 fusion protein can reduce the apoptosis of L02 cells and increase the proliferation rate of L02 cells (P <0.05).