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调查表明甘蔗花叶病在云南发生普遍。电镜检测采自云南6个蔗区主栽品种上的28个甘蔗花叶病病样(分离物),其中25个病样的病叶汁液中观察到弯曲线状的病毒粒体,病叶组织中有风轮状和卷筒状内含体;对这25个分离物进行间接ELISA检测,16个与马铃薯Y病毒属抗血清呈阳性反应,其余呈阴性反应。根据蔗区及其主栽品种的不同,挑选7个分离物进行鉴别寄主测定,结果显示不同分离物鉴别寄主范围和致病性存在明显差异,分离物HH-1有范围最广的鉴别寄主和较强的致病性。克隆并测定HH-1基因组3′末端序列,序列分析发现HH-1的外壳蛋白(CP)基因共864个核苷酸,编码287个氨基酸,与高粱花叶病毒(Sorghum mosaic virus,SrMV)余杭分离物CP氨基酸序列的同源性最高,为97.7%;因此推定HH-1属于SrMV的一个新分离物。
Investigation shows that sugarcane mosaic disease is common in Yunnan. Electron microscopy was used to detect 28 species of sugarcane mosaic disease (isolates) collected from 6 sugarcane growing areas in Yunnan. Among them, 25 viroid diseased leaves showed curved linear virion, diseased leaf tissue There were wind-wheel-shaped and entangled inclusion bodies in these 25 isolates. Indirect ELISA was performed on these 25 isolates, of which 16 were positive with antisera to potato Y virus and the rest were negative. According to the different sugarcane planting areas and its main cultivars, seven isolates were selected to identify the host. The results showed that there were significant differences in the host range and pathogenicity of the isolates. The isolates HH-1 had the most extensive host and Strong pathogenicity. The sequence of HH-1 gene was cloned and sequenced. The sequence analysis showed that the coat protein (CP) gene of HH-1 was 864 nucleotides in length and encoded a protein of 287 amino acids. The CP gene of HH-1 was linked to Sorghum mosaic virus The amino acid sequence of isolate CP has the highest homology of 97.7%. Therefore, it is presumed that HH-1 belongs to a new isolate of SrMV.