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选用来源我国黄河流域至长江流域8省215份芝麻核心种质材料,对其种子中芝麻素(sesamin)和芝麻酚林(sesamolin)含量进行测定,芝麻素平均值5.24mg/g,变异范围为0.88~11.05mg/g,变异系数38.56%,芝麻酚林平均值3.30mg/g,变异范围为0.93~6.96mg/g,变异系数22.68%,二者均符合正态分布,且相关分析表明两者间呈极显著正相关;采用标记-性状关联分析法,进行芝麻素和芝麻酚林与SSR、SRAP、AFLP标记的关联分析。利用GLM模型共检测到33个标记与芝麻素和芝麻酚林极显著(P<0.01)关联,同时与两种成分显著关联的有4个;利用MLM模型共检测到8个显著关联的标记,与两种成分显著关联的分别有4个;其中SSR标记SSI182-3在两种模型中同时极显著关联到芝麻素和芝麻酚林,且解释率较高。该研究将为芝麻功能性成分遗传改良和分子标记研究奠定重要基础。
A total of 215 sesame kernel germplasm collected from 8 provinces in the Yellow River valley of China were used to determine the content of sesamin and sesamolin in their seeds. The average sesamin content was 5.24 mg / g and the variation range was 0.88-11.05mg / g, coefficient of variation 38.56%, average sesame forest 3.30mg / g, variation range 0.93- 6.96mg / g, coefficient of variation 22.68%, both of them are in normal distribution, and the correlation analysis shows that the two The correlation analysis between sesamin and sesamolin and SSR, SRAP and AFLP markers was carried out by using marker-trait correlation analysis. There were a significant (P <0.01) association of 33 markers with sesamin and sesamol in the GLM model, and four markers significantly correlated with the two components. A total of 8 significant correlation markers were detected using the MLM model, There were four significant correlations between the two components. The SSR marker SSI182-3 was significantly associated with both sesamin and sesamol in both models, and the interpretation rate was high. This research will lay a significant foundation for genetic improvement and molecular marker research on functional components of sesame.