论文部分内容阅读
目的:探讨糖尿病时肝组织胰岛素样生长因子-1(IGF-1)基因表达的异常及其与糖尿病外周神经病变的关系。方法:分离解剖出右侧坐骨神经,测定诱发电位出现的波幅(amplitudeofevokedpotentials,AEP)、感觉及运动神经传导速度(sensory/motornerveconductionvelocity,SNCV/MNCV),用四氧嘧啶诱发糖尿病SD大鼠模型。48只糖尿病大鼠按经(胰岛素)控制后血糖水平分成3组:ID-1,2,3组,16只正常大鼠用作对照组。反转录一多聚酶链扩增反应半定量分析坐骨神经IGF-1mRNA含量;酶联免疫吸附法分析组织IGF-1肽含量;诱发肌电图测定坐骨神经电生理指标;观察坐骨神经形态学改变。结果:病程早期(2周,2个月),正常对照组、ID-2组大鼠肝组织IGF-1mRNA含量(1.15±0.090,0.79±0.048,P<0.001;1.17±0.069,0.53±0.023,P<0.001)、肽含量均下降犤(196.66±14.9),(128.2±11.25)μg/g,P<0.001;(196.66±14.9),(74.43±5.33)μg/g,P<0.001犦出现在相应糖尿病组大鼠坐骨神经电生理指标异常之前,程度均随糖尿病控制状态而异,且随病程进一步逐渐下降犤IGF-1mRNA(0.71±0.024)～(0.47±0.021);IGF-1肽(114.35±8.09)～(64.58±3.89)μg/g,P<0.001犦。血清IGF-1呈一致性下降(r=0.99,P<0.001)。其变化与坐骨神经功能改变(感觉神经:r=0.54,P<0.05,
Objective: To investigate the abnormal expression of insulin-like growth factor-1 (IGF-1) gene and its relationship with diabetic peripheral neuropathy in diabetic patients. Methods: The right sciatic nerve was dissected, the amplitude of evoked potential (AEP), sensory / motor nerve conduction velocity (SNCV / MNCV) were measured and alloxan-induced SD rats were induced. The blood glucose levels of 48 diabetic rats controlled by menstruation (insulin) were divided into 3 groups: ID-1,2,3 group and 16 normal rats as the control group. Reverse transcription-polymerase chain reaction amplification semi-quantitative analysis of sciatic nerve IGF-1mRNA content; enzyme-linked immunosorbent assay IGF-1 peptide content; induced EMG electrophysiological indicators sciatic nerve; observe sciatic nerve morphological changes. Results: In the early course of disease (2 weeks, 2 months), the content of IGF-1mRNA in liver tissue of normal control group and ID-2 group were 1.15 ± 0.090,0.79 ± 0.048, P <0.001; 1.17 ± 0.069,0.53 ± 0.023, (P <0.001), and the peptide contents decreased (196.66 ± 14.9), (128.2 ± 11.25) μg / g, P <0.001; (196.66 ± 14.9) and (74.43 ± 5.33) μg / g respectively The levels of IGF-1mRNA (0.71 ± 0.024) ~ (0.47 ± 0.021) and IGF-1peptide (114.35 ±) in the corresponding diabetic rats before and after the sciatic nerve electrophysiological indexes were different with the course of diabetes control, 8.09) ~ (64.58 ± 3.89) μg / g, P <0.001 犦. Serum IGF-1 was consistently decreased (r = 0.99, P <0.001). The changes and changes of sciatic nerve function (sensory nerve: r = 0.54, P <0.05,