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目的:研究不同浓度的秋水仙碱对斑马鱼肝脏和鳃组织超氧化物歧化酶(SOD)和Na+-K+-ATPase活性的影响,为以斑马鱼的酶类作为观测指标进行药物安全性评价提供依据。方法:在预实验的基础上将斑马鱼暴露于秋水仙碱5个浓度组中进行96 h急性毒性试验,计算其半数致死浓度(LC50)。此外,设置3个浓度(0.67,1.70,4.26 mg.L-1),进行21 d慢性毒性试验,期间每7 d测定斑马鱼的肝脏和鳃中的SOD和Na+-K+-ATPase活性。结果:秋水仙碱对斑马鱼的LC50为16.90 mg.L-1。随秋水仙碱浓度增大和染毒时间延长,斑马鱼鳃中SOD和Na+-K+-ATPase活性均显著受到抑制;肝脏SOD活性增加,Na+-K+-AT-Pase活性总体呈现抑制趋势。结论:斑马鱼肝脏和鳃的SOD及Na+-K+-ATPase对毒物敏感,可作为观测指标用于评价药物的毒性。
OBJECTIVE: To study the effects of different concentrations of colchicine on the activities of superoxide dismutase (SOD) and Na + -K + -ATPase in the liver and gill of zebrafish, and provide the safety evaluation of the zebrafish as an observation index in accordance with. Methods: Based on the preliminary experiments, the zebrafish were exposed to 5 concentrations of colchicine for 96 h acute toxicity test and the LC50 was calculated. In addition, three concentrations (0.67,1.70,4.26 mg.L-1) were set up for a 21-day chronic toxicity test. The activities of SOD and Na + -K + -ATPase in the liver and gills of zebrafish were determined every 7 days. Results: The LC50 of colchicine to zebrafish was 16.90 mg.L-1. The activities of SOD and Na + -K + -ATPase in zebra gills were significantly inhibited with the increase of colchicine concentration and exposure time. The activity of SOD in liver increased and the activity of Na + -K + -AT-Pase tended to be inhibited. Conclusion: SOD and Na + -K + -ATPase in the liver and gills of zebrafish are sensitive to poison, which can be used as an indicator to evaluate the toxicity of drugs.