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目的探讨卵巢上皮性癌组织及外周血中septin-9蛋白水平检测的临床意义。方法采用酶联免疫吸附分析法分别测定137例卵巢上皮性癌患者、12例卵巢交界性肿瘤患者、10例卵巢良性肿瘤患者、41例盆腔良性疾患病人和58例健康人外周血血浆中septin-9蛋白水平。采用免疫组织化学染色方法分别测定54例卵巢上皮性癌患者、8例卵巢交界性囊腺瘤患者和8例卵巢囊腺瘤患者肿瘤组织中septin-9蛋白表达水平。结果卵巢上皮性癌患者和盆腔良性疾患患者血浆中的septin-9蛋白水平(OD450=0.35±0.09;0.36±0.09)显著高于健康对照组(OD450=0.31±0.12)(P=0.002,P=0.007)。卵巢上皮性癌患者外周血中septin-9水平,有肿瘤家族史组高于否认肿瘤家族史组(P=0.040),发生远处转移组高于未发生远处转移组(P=0.025)。血浆中的septin-9蛋白水平与不同临床病理分期、分化、淋巴转移差异无统计学意义。组织中septin-9阳性表达率,卵巢上皮性癌组织为59.3%(32/54),卵巢交界性肿瘤组织为50%(4/8),卵巢良性肿瘤组织为0(0/8),各组间比较差异有统计学意义(P=0.007)。结论卵巢上皮性癌患者外周血中septin-9蛋白水平显著高于健康人群,盆腔良性疾病组患者外周血中septin-9蛋白水平显著高于健康人群。Septin-9与卵巢上皮性癌远处转移密切相关。卵巢上皮性癌组织、卵巢交界性肿瘤组织中septin-9阳性表达率显著高于卵巢良性肿瘤组织。
Objective To investigate the clinical significance of detecting septin-9 protein in ovarian epithelial carcinoma and peripheral blood. Methods Serum levels of septin-1 in 137 patients with epithelial ovarian cancer, 12 patients with ovarian borderline tumor, 10 patients with benign ovarian tumor, 41 patients with pelvic benign disease and 58 healthy controls were detected by enzyme-linked immunosorbent assay (ELISA) 9 protein level. Immunohistochemical staining was used to determine the expression of septin-9 in 54 cases of ovarian epithelial carcinoma, 8 cases of ovarian borderline cystadenoma and 8 cases of ovarian cystadenoma. Results The serum levels of septin-9 in patients with epithelial ovarian cancer and patients with pelvic benign disease were significantly higher than those in healthy controls (OD450 = 0.35 ± 0.09; 0.36 ± 0.09, P = 0.002, P = 0.007). The level of septin-9 in the peripheral blood of patients with epithelial ovarian cancer was higher than that of family history of cancer (P = 0.040), distant metastasis was higher than that of no distant metastasis (P = 0.025). Plasma septin-9 protein levels and different clinical pathological stages, differentiation, lymph node metastasis was no significant difference. The positive rate of septin-9 was 59.3% (32/54) in epithelial ovarian cancer, 50% (4/8) in borderline ovarian tumor and 0 (0/8) in benign ovarian tumor, The difference between the two groups was statistically significant (P = 0.007). Conclusion The level of septin-9 protein in peripheral blood of patients with epithelial ovarian cancer is significantly higher than that of healthy people. The serum level of septin-9 in patients with pelvic benign disease is significantly higher than that of healthy people. Septin-9 is closely related to the distant metastasis of ovarian epithelial carcinoma. The positive rate of septin-9 in epithelial ovarian cancer and borderline ovarian tumor was significantly higher than that in benign ovarian tumor.