帕珠丸对慢性乙醇性肝损伤大鼠PPAR-α1与AMPK-α1的影响

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目的:观察帕珠丸对乙醇性肝损伤大鼠肝组织中过氧化物酶体增殖物激活受体α1(PPAR-α1)及腺苷酸活化蛋白激酶α1(AMPK-α1)表达水平的影响及探讨其对肝脏的保护作用。方法:将78只纯系雄性SD大鼠随机分成正常对照组、模型对照组、联苯双酯组、帕珠丸低、中、高剂量组6组。模型组、联苯双酯组、帕珠丸低、中、高剂量组分别给予56%红星二锅头酒10 mL·kg-1灌胃;正常组给予10 mL·kg-1生理盐水灌胃,每天上午1次,连续灌胃12周,复制乙醇性肝损伤模型。造模成功后,帕珠丸低、中、高剂量组分别给予10 mL·kg-1帕珠丸混悬液灌胃,剂量依次为0.05,0.10,0.20 g·kg-1;联苯双酯组给予10 mL·kg-1联苯双酯滴丸混悬液灌胃,剂量为0.003 g·kg-1;正常对照组、模型对照组分别给予同体积生理盐水灌胃,各组每天上午灌胃1次,连续灌胃3周。检测血清丙氨酸转氨酶(ALT)、天门冬氨酸转氨酶(AST)的活性及甘油三酯(TG)的水平,并检测肝组织中PPAR-α1 mRNA、AMPK-α1 mRNA的表达水平。结果:与正常组比较,模型组大鼠血清肝脏湿重和肝指数以及ALT,AST,TG,均显著升高(P<0.05),模型组大鼠肝组织中PPAR-α1 mRNA,AMPK-α1 mRNA显著降低(P<0.01)。与模型组比较,帕珠丸各剂量组与联苯双酯组肝脏湿重和肝指数及ALT,AST,TG均显著降低(P<0.05);与模型组比较,帕珠丸各剂量组与联苯双酯组PPAR-α1 mRNA,AMPK-α1 mRNA均显著升高(P<0.05)。结论:帕珠丸可上调肝组织内PPAR-α1mRNA,AMPK-α1 mRNA的表达,提示该药对乙醇性肝病有一定的保护作用。 Objective: To observe the effect of Pazhu pill on the expression of peroxisome proliferator-activated receptor α1 (PPAR-α1) and adenosine-activated protein kinase α1 (AMPK-α1) in rats with alcoholic liver injury To explore its protective effect on the liver. Methods: Seventy-eight male Sprague Dawley rats were randomly divided into 6 groups: normal control group, model control group, bifendate group and Paizhuwan low, medium and high dose group. The model group, the bifendate group and the peizhu pill low, middle and high dose group were given 56% red star Erguotou liquor 10 mL · kg-1 orally; the normal group was given 10 mL · kg-1 saline, every day The morning 1, continuous gavage for 12 weeks, replicate alcohol liver injury model. After successful modeling, Pazuzhu low, medium and high dose groups were given 10 mL · kg-1 Pazhu ball suspension orally, the dose was 0.05,0.10,0.20 g · kg-1; bifendate Group was given 10 mL · kg-1 bifendate drip suspension gavage, a dose of 0.003 g · kg-1; normal control group, model control group were given the same volume of normal saline gavage, each group morning irrigation Stomach 1, continuous gavage for 3 weeks. Serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and triglyceride (TG) were measured. The expression of PPAR-α1 mRNA and AMPK-α1 mRNA in liver tissue were detected. Results: Compared with the normal group, the serum liver wet weight, liver index, ALT, AST and TG in the model group were significantly increased (P <0.05). The levels of PPAR-α1 mRNA and AMPK-α1 mRNA was significantly lower (P <0.01). Compared with the model group, the liver wet weight, liver index, ALT, AST and TG in each dose group and the bifendate group were significantly decreased (P <0.05). Compared with the model group, PPAR-α1 mRNA and AMPK-α1 mRNA in bifendate group were significantly increased (P <0.05). Conclusion: Peizhu pill can up-regulate the expression of PPAR-α1mRNA and AMPK-α1 mRNA in liver tissue, suggesting that the drug has certain protective effect on alcoholic liver disease.
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