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本研究以不同槟榔品种的幼嫩叶片及花序为外植体成功实现体细胞胚胎发生及植株再生。试验结果显示,不同品种及外植体均以毒莠定为最佳愈伤组织诱导剂。诱导所获愈伤组织需连续用生长素浓度由高到低的一系列培养基进行继代培养才能不断生长并诱导体细胞胚胎。当愈伤组织最终转移入无激素MS配养基后可观察到体细胞胚胎发生。胚胎萌发以附加20μmBA的MS配养基效果为最佳。之后,将萌发的胚胎转入含有5μmBA的MS液体配养基以促使其快速生长和发育。试验中我们注意到,除了培养周期长短之外,不同品种在愈伤组织诱导,体细胞胚胎发生,以及小植株发育上均无任何差异。当小植株长出2-4片叶并发育出健壮根系时即可移栽到营养袋中育苗。
In this study, somatic embryos and plantlets were successfully regenerated with young leaves and inflorescences of different areca varieties. The results showed that all varieties and explants were treated with piclorast as the best callus induction agent. The induced callus is continuously subcultured with a series of media with high and low auxin concentrations to induce somatic embryos. Somatic embryogenesis is observed when the callus is finally transferred into hormone-free MS media. Embryo germination with an additional 20 μmBA of MS medium is the best. Afterwards, the germinated embryos were transferred to MS liquid medium containing 5 uM BA to facilitate their rapid growth and development. In the experiment, we noticed that there was no difference in callus induction, somatic embryogenesis and plantlet development among different cultivars except for the length of the cultivation period. When the plantlets grow 2-4 leaves and develop strong roots can be transplanted to nutrition bags nursery.