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目的:研究microRNA-192基因表达降低与恶性黑素瘤A375细胞增殖及凋亡的关系.方法:除空白对照组外,运用脂质体介导法将空载体和为microRNA-192设计的small interfering RNA(siRNA)转染到恶性黑素瘤A375细胞中,并利用实时定量PCR检测microRNA-192的表达.通过形态学观察、细胞计数法、3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)比色法以及流式细胞免疫学等方法,分析空白对照组、空载体对照组(lipofectamine2000)和实验组恶性黑素瘤A375细胞的增殖和早期凋亡情况.结果:空白对照组和空载体对照组之间相比较,细胞增殖和凋亡情况几乎一致,差异无统计学意义.与空载体对照组相比,实验组存活的恶性黑素瘤A375细胞的活性和数量明显降低,此现象并随着siRNA浓度的提高而愈发明显.另外,实验组恶性黑素瘤A375细胞细胞凋亡指数较空载体对照组明显升高.结论:通过下调恶性黑素瘤细胞中microRNA-192基因表达可能会有效地抑制恶性黑素瘤的生长并促使其细胞凋亡.“,”Objective:We assessed whether microRNA-192 deficiency influences the proliferation and apoptosis in A375 melanoma cell line.Methods:A375 melanoma cells were transfected with lipofectamine alone or small interfering RNA(MicroRNA-1192 siR NA)-loaded lipofectamine.A group of untreated cells served as an additional normal control The expression of microRNA-192 was analyzed with real-time PCR.Cell proliferation and early apoptosis were assessed with inverted microscope,MTT assay,and flow cytometry.Result:There were no significant differences in proliferation and apoptosis between normal controls and cells-treated with lipofectamine alon~ In comparison with lipofectamine alone,siRNA treatment induced significant reduction in cell activity and the number of the A375 melanoma cells in a dose-dependent manner.Additionally,transfection with MicroRNA-1192 siRNA dramatically increased apoptosis index.Conclusion:Downregulation of microRNA-192 can effectively inhibit the proliferation and accelerate the apoptosis in A375 melanoma cell line.