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目的:了解帕金森病大鼠模型纹状体内谷氨酸(glutamate,Glu)、γ-氨基丁酸(gama-aminobutyric acid,GABA)和多巴胺(dopamine,DA)之间的关系,从而进一步探讨帕金森病的发病机制。方法:动物分为溶剂对照组、假手术组和帕金森模型组。大脑右侧黑质致密部和前脑内侧束两点注射6-羟基多巴胺(6-hydroxydopamine,6-OHDA)建立帕金森病大鼠模型,溶剂对照组注入生理盐水,假手术组不注射任何药物,采用脑微透析术于建模后第3,4,5,6周连续动态透析大鼠毁损侧纹状体,结合高效液相色谱(HPLC)动态监测各组谷氨酸、GABA和多巴胺的变化。结果:(1)PD组纹状体内多巴胺含量到第5周仅为溶剂对照组和假手术组的1/5;(2)谷氨酸含量随建模时间逐渐升高,到第6周PD组是溶剂对照组和假手术组的1倍以上;(3)GABA含量呈下降趋势,到第6周约降至溶剂对照组、假手术组的1/2。结论:帕金森病大鼠模型纹状体内谷氨酸的变化与多巴胺分泌可能存在某种联系;GABA含量随建模时间的增加而下降。
Objective: To investigate the relationship between glutamate (Glu), gama-aminobutyric acid (GABA) and dopamine (DA) in the striatum of Parkinson’s disease rat model, The pathogenesis of Parkinson’s disease. Methods: Animals were divided into solvent control group, sham operation group and Parkinson’s model group. The rat model of Parkinson’s disease was established by injection of 6-hydroxydopamine (6-hydroxydopamine, 6-OHDA) into the right substantia nigra pars compacta and the medial forebrain. Normal saline was injected into the solvent control group and no drug was injected into the sham operation group . The rats were subjected to dynamic microdialysis on the 3rd, 4th, 5th, 6th week after cerebral microdialysis. The striatum was destroyed and the contents of glutamate, GABA and dopamine in each group were dynamically monitored by high performance liquid chromatography (HPLC) Variety. Results: (1) The content of dopamine in the striatum of PD group was only 1/5 of the solvent control group and the sham operation group in the fifth week; (2) The content of glutamic acid gradually increased with the modeling time, and the PD Group was more than 1 times the solvent control group and sham operation group; (3) GABA content showed a downward trend, about 6 weeks reduced to solvent control group, sham group 1/2. CONCLUSIONS: There may be some connection between the change of glutamate in striatum and dopamine secretion in Parkinson’s disease rat model. The content of GABA decreased with the increase of modeling time.