论文部分内容阅读
目的:探讨四制香附贴膏灸剂对缩宫素所致痛经模型大鼠血液流变性的影响,并初步探究其体外透皮特性。方法:SD大鼠随机分为空白组、模型组、四制香附贴膏灸剂组(贴膏灸剂组)及四制香附石油醚部位浸膏组(浸膏组)。通过灌胃己烯雌酚增敏,观察腹腔注射缩宫素后30 min内大鼠扭体发生的潜伏期及次数,测试各组大鼠的全血黏度和血浆黏度;采用改良的Franz扩散池,以小鼠离体皮肤为渗透屏障进行体外透皮试验,利用HPLC同时测定α-香附酮和香附烯酮的体外透皮渗透量,分别对四制香附贴膏灸剂组和不加发热辅助材料的四制香附贴膏剂组进行研究。结果:贴膏灸剂组和浸膏组均显著延长大鼠扭体反应发生的潜伏期,显著减少30 min内大鼠扭体发生次数,且贴膏灸剂组优于浸膏组;贴膏灸剂组和浸膏组均可显著降低痛经模型大鼠的全血黏度和血浆黏度,贴膏灸剂组更佳。四制香附贴膏灸剂中香附烯酮和α-香附酮24 h内的渗透速率分别为58.58,45.42μg·cm~(-2)·h~(-1),均高于不加发热辅助材料的贴膏剂组,透皮行为符合Higuchi方程。结论:四制香附贴膏灸剂可显著提高四制香附抗痛经有效成分的透皮释药性能,对大鼠痛经模型有良好的镇痛作用,可显著改善痛经大鼠的血液流变性。
OBJECTIVE: To investigate the effect of Si Xiangxiang paste plaster on the hemorheology of rats with dysmenorrhea caused by oxytocin and to explore its in vitro transdermal properties. Methods: The SD rats were randomly divided into blank group, model group, SXSG plaster and moxibustion group (patch plaster and moxibustion group) By intragastric administration of diethylstilbestrol, the latency and number of writhing in rats within 30 min after intraperitoneal injection of oxytocin were observed, and the whole blood viscosity and plasma viscosity of each group were measured. Using the modified Franz diffusion cell, In vitro skin permeation test was carried out in vitro. The in vitro transdermal permeation of α-ketones and flavones was measured by HPLC. Four-system Xiangxiang paste group to study. Results: The plaster and moxibustion group and the extract group both prolonged the latent period of rat writhing reaction and significantly reduced the number of writhing in rats within 30 min, and the plaster moxibustion group was better than the extract group. The agent group and the extract group can significantly reduce the rat model of dysmenorrhea blood viscosity and plasma viscosity, plaster moxibustion group better. The permeation rates of clovene and α-ketoconazole in 24 h were 58.58 and 45.42 μg · cm -2 · h -1, respectively, all higher than those without With heat-assisted material patch group, transdermal behavior in line with Higuchi equation. CONCLUSION: SIHXI plaster can significantly improve the transdermal drug delivery of the active ingredients of SJXT and relieve dysmenorrhea in rats, and can significantly improve the blood rheology of dysmenorrhea rats .