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目的观察不同级别和不同分子亚型乳腺癌中HIF-1α的表达,探讨HIF-1α在乳腺癌中表达的意义。方法收集乳腺癌病例105例和正常对照17例,根据2012 WHO乳腺肿瘤中的标准进行分级,通过免疫组化检测各级中HIF-1α、ER、PR和HER-2的表达并根据常用的四分型法进行分型。结果 HIF-1α在正常乳腺和各级乳腺癌中的表达差异有统计学意义(P<0.05),其中Ⅱ级、Ⅲ级乳腺癌中的HIF-1α表达阳性率(76.19%和80.00%)高于正常乳腺和Ⅰ级乳腺癌(17.65%和23.53%)(P<0.01),正常乳腺(17.65%)、Ⅰ级(23.53%)之间以及Ⅱ级(76.19%)、Ⅲ级(80.00%)之间的表达无差异(P>0.05)。105例乳腺癌中各分子亚型HIF-1α表达的阳性率无差别,且均未检测到HIF-1α的表达与ER、PR和HER-2的相关性(P>0.05)。但不同级别乳腺癌中各分子亚型分布情况有差异(P<0.05)。结论 HIF-1α可能在恶性度较高的乳腺癌中起着更重要的调控作用,而HIF-1α与乳腺癌分子亚型的关系仍需进一步探讨,为HIF-1α为靶点的个体化治疗奠定基础。
Objective To observe the expression of HIF-1α in different grades and subtypes of breast cancer, and to explore the significance of HIF-1α expression in breast cancer. Methods 105 cases of breast cancer and 17 normal controls were collected and classified according to the criteria in 2012 WHO breast neoplasms. The expression of HIF-1α, ER, PR and HER-2 at all levels was detected by immunohistochemistry. Typing method for typing. Results There was significant difference in the expression of HIF-1α between normal breast and breast cancer (P <0.05). The positive rate of HIF-1α expression in grade Ⅱ and Ⅲ breast cancer was 76.19% and 80.00% respectively (17.65% vs 23.53%), normal breast (17.65%), grade Ⅰ (23.53%), grade Ⅱ (76.19%), grade Ⅲ (80.00%), There was no difference between the two groups (P> 0.05). The positive rate of HIF-1α expression in 105 cases of breast cancer was not different, and no correlation was found between the expression of HIF-1α and ER, PR and HER-2 (P> 0.05). However, the different subtypes of breast cancer in different distribution (P <0.05). Conclusions HIF-1α may play a more important role in the regulation of breast cancer. However, the relationship between HIF-1α and molecular subtypes of breast cancer remains to be further explored. Individualized HIF-1α targets Lay the foundation.