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Based on a multi-proxy investigation into the deep core of the Cuoe Lake in the middle of Tibetan Plateau, a 2.8 Ma paleoclimatic and paleoenvironmental evolution is reconstructed. The result of magnetic stratum indicates that the lake basin was formed at about 2.8 MaBP, while the multi-proxy analyses of lithology, grain size, magnetic susceptibility and geochemical elements reveal that there have been three major environmental evolution stages and at least two intensive uplifts of the Tibetan Plateau in the lake basin area, i.e. during 2.8-2.5 MaBP, the lake basin came into being as a result of the disaggregation of the planation surface and rapid rising of the Tibetan Plateau. During 2.5-0.8 MaBP, with gradual uplift of the Tibetan Plateau, the environment of this area was more effectively controlled by the climatic cycle of the alternative glacial-interglacial stages. After 0.8 MaBP, the middle part of the Plateau accelerated its uplift and entered cryoshere.