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目的:通过含铜宫内节育器(Cu-IUD)的Cu在家兔体内的血药浓度变化,为临床研究人体内Cu-IUD Cu释放过程和Cu~(2+)代谢规律提供方法和依据。方法:在家兔左侧子宫近阴道处作一切口,分别朝阴道方向插入改造后的人用TCu380A(Cu表面积约317mm~2,高剂量组)、TCu220C(Cu表面积约157 mm~2,低剂量组)和无铜支架(对照组),并将其固定于子宫壁上,在植入32周内的不同时间点,通过原子吸收火焰法测定家兔血清和局部宫腔液的Cu~(2+)浓度,分析统计Cu~(2+)的吸收代谢规律。结果:TC220C组和TCu380A组家兔在放置IUD不同时间点,局部宫腔液维持在高水平(P<0.01),同时血清Cu~(2+)浓度则维持在较低且恒定的范围,各组家兔血清Cu~(2+)水平在各放置时间点均无明显差异(P>0.05)。结论:Cu-IUD各组家兔在各时间点血药浓度均维持在较低且稳定的范围,提示其长期应用不会出现体内蓄积。
OBJECTIVE: To provide a method and a basis for clinical study of Cu-IUD Cu release process and Cu 2+ metabolism in Cu patients by Cu-IUD copper concentration change in rabbits . Methods: All the rabbits were treated with transvaginal approach in the left uterus of the rabbits. The modified TCu380A (Cu surface area of about 317mm ~ 2, high dose group), TCu220C (Cu surface area of 157mm ~ 2, Dose group) and copper-free stent (control group), and fixed on the uterine wall. At different time points within 32 weeks after implantation, the contents of Cu ~ (superscript +) in rabbit serum and local uterine fluid were measured by flame atomic absorption spectrometry 2+) concentration, analysis of absorption and metabolism of Cu ~ (2+). Results: In the TC220C group and the TCu380A group, the local intrauterine fluid was maintained at a high level (P <0.01) and the Cu2 + concentration in the serum remained at a low and constant range at different time points after IUD placement There was no significant difference in the level of Cu 2+ between the two groups (P> 0.05). CONCLUSION: The plasma concentrations of Cu-IUD in all the groups maintained at a low and stable range at various time points, suggesting that they will not accumulate in vivo in long-term use.