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目的:探讨子宫颈上皮内瘤变(CIN)及宫颈鳞癌组织中FHIT蛋白的异常表达及意义。方法:采用免疫组化Powervision法检测40例正常宫颈组织、50例CIN及41例宫颈浸润鳞癌标本中FHIT蛋白的表达情况。结果:①FHIT蛋白在正常宫颈组织、CIN及宫颈癌中的低表达率分别为7.50%、34.00%、68.29%,三者比较差异有统计学意义(P<0.01)。②FHIT蛋白在CINⅠ及CINⅡ～Ⅲ中的低表达率分别为12.50%和44.12%,二者比较差异有统计学意义(P<0.05)。③FHIT蛋白在宫颈鳞癌不同临床分期中的低表达率分别为58.82%和100.00%,二者比较差异有统计学意义(P<0.05)。④FHIT蛋白在伴有淋巴结转移者与无淋巴结转移者中的低表达率分别为87.50%和52.00%,二者比较差异有统计学意义(P<0.05)。结论:①FHIT蛋白在正常宫颈组织、CIN、宫颈癌中随着宫颈病变的进展其阳性表达呈明显减少趋势,提示FHIT可作为临床监测CIN转归的分子生物学指标。②FHIT蛋白的表达与宫颈鳞癌、淋巴结转移及临床分期相关,提示它可作为预测宫颈鳞癌患者预后的参考依据。
Objective: To investigate the abnormal expression of FHIT protein in cervical intraepithelial neoplasia (CIN) and cervical squamous cell carcinoma and its significance. Methods: Immunohistochemistry was used to detect the expression of FHIT protein in 40 cases of normal cervical tissue, 50 cases of CIN and 41 cases of cervical squamous cell carcinoma. Results ① The low expression rates of FITC protein in normal cervical tissue, CIN and cervical cancer were 7.50%, 34.00% and 68.29%, respectively, with significant difference (P <0.01). ② The low expression rates of FITC protein in CINⅠ and CINⅡ ~ Ⅲ were 12.50% and 44.12%, respectively, with significant difference (P <0.05). ③ The low expression rates of FITC protein in different clinical stages of cervical squamous cell carcinoma were 58.82% and 100.00%, respectively, with significant difference (P <0.05). ④ The low expression rates of FITC protein in patients with and without lymph node metastasis were 87.50% and 52.00%, respectively, with significant difference (P <0.05). Conclusions: ①The positive expression of FITC protein in normal cervical tissue, CIN and cervical cancer with the progression of cervical lesions shows a decreasing trend, suggesting that FHIT can be used as a molecular biological indicator for clinical monitoring of CIN. ②FHIT protein expression and cervical squamous cell carcinoma, lymph node metastasis and clinical stage, suggesting that it can be used as a prognostic reference for cervical squamous cell carcinoma prognosis.