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以固定化胆碱氧化酶(EC 5896)复合H2O2电极构成电流型酶电极生物传感分析仪,酶电极测定线性范围:0 mg.L-1~200 mg.L-1,精度RSD<1.5%,响应时间:40 s,固定化胆碱氧化酶膜使用寿命大于60 d,实际测定氯化琥珀胆碱注射液中氯化胆碱含量回收率:100.3%~102.3%,采用酶电极法测定氯化琥珀胆碱注射液中氯化胆碱含量具有专一性高、成本低、分析速度快等优点。
The current-type enzyme electrode biosensor was constructed with immobilized choline oxidase (EC 5896) composite H2O2 electrode. The linear range of enzyme electrode was 0 mg.L-1 ~ 200 mg.L-1 with RSD <1.5% , The response time: 40 s, immobilized choline oxidase membrane service life of more than 60 d, the actual determination of choline chloride choline chloride injection choline chloride recovery: 100.3% ~ 102.3%, using the enzyme electrode method for the determination of chlorine Choline chloride injection choline chloride content with high specificity, low cost, fast analysis and so on.