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目的:研究丹参注射液对槟榔提取液(aqueousarecanutextract,AANE)所导致的血管内皮细胞(endothe-lialcell,EC)损伤的保护作用,旨在为丹参注射液治疗口腔黏膜下纤维性变(oralsubmucousfibrosis,OSF)提供理论依据。方法:体外培养人脐静脉EC,分为保护组及预防组2组,分别在AANE作用前后将EC与丹参注射液共同培养,MTT法检测EC增殖,放免法检测EC分泌内皮素(1endothelin-1,ET-1)量的变化。结果:①在AANE干预之后将丹参注射液与EC共同培养,与对照组相比,EC增殖情况明显增加(t=-2.805,p<0.05),分泌ET-1的量显著减少(t=2.965,p<0.05)。②在AANE干预之前先将丹参注射液与EC共同培养,发现EC增殖(t=2.701,p>0.05)分泌ET-1的量(t=-1.541,p>0.05)与对照组相比差异无统计学意义。结论:丹参注射液能有效抑制AANE导致的EC损伤,使EC分泌ET-1减少,其机制可能通过ET-1这个靶标来发挥抗纤维化作用。
OBJECTIVE: To study the protective effect of Salvia miltiorrhiza injection on the damage of vascular endothelial cells (ECs) induced by aqueous extract of betel nut extract (AANE) in order to treat oral submucous fibrosis (OSF) for Danshen injection. ) Provide theoretical basis. METHODS: Human umbilical vein EC was cultured in vitro and divided into two groups: protection group and prevention group. EC was incubated with Danshen injection before and after AANE treatment. EC proliferation was detected by MTT assay, and EC secretion of endothelin was measured by radioimmunoassay (1endothelin-1). ET-1) changes in quantity. RESULTS: 1 After intervention of AANE, Danshen injection was co-cultured with EC. Compared with the control group, EC proliferation was significantly increased (t=-2.805, p<0.05), and the amount of secreted ET-1 was significantly decreased (t=2.965). , p<0.05). 2 Before the intervention of AANE, we first co-cultured Danshen injection with EC, and found that the amount of ET-1 secreted by EC proliferation (t=2.701, p>0.05) (t=-1.541, p>0.05) was not different from the control group. Statistical significance. Conclusion: Salvia miltiorrhiza injection can effectively inhibit the EC damage caused by AANE and reduce the secretion of ET-1 by EC. The mechanism may be through the anti-fibrosis effect of ET-1.