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RNA编辑是陆生植物叶绿体转录后基因表达调控的一种重要方式。为进一步探讨单子叶植物RNA编辑功能及其发生机制,本研究通过生物信息学方法,对二穗短柄草叶绿体的81个蛋白编码基因的RNA编辑位点进行了预测和鉴定分析,结果发现78个基因存在编辑位点,共检测到176个编辑位点,都是C到U的转换。其中ndhB最多,为14个编辑位点。为验证预测结果,利用blast工具比对了NCBI的二穗短柄草EST数据库,最终确定存在于17个基因中的34个编辑位点是真实存在的,其中19个为沉默编辑,15个为有效编辑。与5个不同单子叶禾本科物种18个蛋白编码基因的RNA编辑位点的比较发现,在rpoB-206位点,只有二穗短柄草发生了编辑,且只有ndhD-295(293)为部分编辑位点。
RNA editing is an important way of terrestrial plant chloroplast gene transcription regulation. In order to further explore the function and mechanism of RNA editing in monocotyledons, we predicted and identified the RNA editing sites of 81 protein-coding genes in Brachypodium distachyon by bioinformatics method. The results showed that 78 A total of 176 editing sites were detected in the presence of editing sites, all of which were C to U conversion. Which ndhB up to 14 editing sites. In order to verify the prediction results, the NCBI EST database of Brassicapsis anguillarum was compared with the blast tool, and finally 34 ESTs existing in 17 genes were found to be true, of which 19 were silent editors and 15 were Effective editing. Comparisons with RNA editing sites for 18 protein-coding genes from five different monocotyledonous species revealed that only the Brachypodium distachyon was edited at the rpoB-206 site and only ndhD-295 (293) was part of Edit the site.