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目的探讨氧化应激反应在自体移植静脉术后内膜增生中的作用。方法将雌性Sprague-Dawley(SD)大鼠70只按随机分组方法分为两组:实验组(60只),分为移植术后1d、1周、2周、4周、6周和2个月6个时间点,每个时间点10只,建立大鼠自体移植静脉模型,以上6个时间切取移植静脉;对照组(10只),直接切取同样长度的颈外静脉。应用计算机图象分析仪计算新生内膜面积/中膜面积(neointima to tunica media area,I/M);应用免疫组织化学方法检测核转录因子-κB(nuclear factor-kappa B,NF-κB)和铜锌超氧化物歧化酶(copper zinc superoxidedismutase,CuZnSOD);用比色法检测大鼠血清中丙二醛(malondialdehyde,MDA)的含量。结果对照组大鼠NF-κB、CuZnSOD有少量表达。实验组大鼠移植术后NF-κB的表达随着时间的增加逐渐增加,移植术后2周达到高峰,维持1个月左右后逐渐下降,2个月时接近基础水平;CuZnSOD表达逐渐升高,移植术后1周左右达到高峰,持续2～3周逐渐下降至正常水平。对照组血清中MDA含量为4.966±1.346nmol/ml,实验组移植术后大鼠血清中MDA的含量急剧升高,移植术后1d达到高峰(21.161±2.174nmol/ml),然后逐渐下降,2个月时接近正常水平(6.208±2.908nmol/ml),两组比较差异有统计学意义(P<0.05);对照组I/M为0.2096±0.0253,实验组移植术后1周I/M为0.6806±0.0737,4周达高峰(1.4527±0.0824),6周为1.0353±0.0656,2个月时为0.9583±0.0516,两组差异有统计学意义(P<0.05)。结论自体静脉移植后内皮细胞损伤导致氧化应激反应,通过激活NF-κB放大炎症反应,在引发移植静脉再狭窄过程中起重要作用。
Objective To investigate the role of oxidative stress in neointimal hyperplasia after autologous vein graft. Methods Seventy female Sprague-Dawley (SD) rats were randomly divided into two groups: experimental group (60 rats), which were divided into 1, 1, 2, 4, 6 and 2 weeks after transplantation Month 6 time points, at each time point 10, the establishment of autologous vein graft model in rats, the above 6 time cut vein graft; control group (10), the same length of the external jugular vein directly cut. The neointima to tunica media area (I / M) was calculated by computer image analyzer. The expression of nuclear factor-kappa B (NF-κB) was detected by immunohistochemistry Copper zinc superoxide dismutase (CuZnSOD). The content of malondialdehyde (MDA) in rat serum was detected by colorimetry. Results In the control group, there was a small amount of expression of NF-κB and CuZnSOD. The expression of NF-κB increased gradually with the increase of time in experimental group, reaching the peak at 2 weeks after transplantation, and gradually decreased after 1 month and reached the basic level at 2 months. The expression of CuZnSOD gradually increased , Peaked at about 1 week after transplantation, and gradually decreased to normal level for 2 to 3 weeks. The content of MDA in the serum of the control group was 4.966 ± 1.346nmol / ml. The content of MDA in the serum of the experimental group after transplantation increased sharply (21.161 ± 2.174nmol / ml) on the 1st day after transplantation, and then decreased gradually. 2 (P <0.05). The I / M of the control group was 0.2096 ± 0.0253, and the I / M of the experimental group at 1 week after transplantation was significantly higher than that of the control group (6.208 ± 2.908 nmol / 0.6806 ± 0.0737, peaked at 4 weeks (1.4527 ± 0.0824), 1.0353 ± 0.0656 at 6 weeks, and 0.9583 ± 0.0516 at 2 months. The difference between the two groups was statistically significant (P <0.05). Conclusion The endothelial cell injury resulted in oxidative stress response after autologous vein transplantation. It may play an important role in triggering the restenosis of vein graft by activating NF-κB to amplify the inflammatory reaction.