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目的:观察环氧合酶2(COX-2)在前列腺炎性疼痛大鼠前列腺组织中的表达变化,探讨小金丸治疗前列腺炎性疼痛可能的作用机制。方法:60只雄性Wistar大鼠,随机取10只用作空白组,与其前列腺腹叶注入无菌注射用水,其余50只注射弗氏完全佐剂(CFA)制备前列腺炎性疼痛模型,术毕3d后,随机分为模型组、塞来昔布胶囊组、小金丸高、中、低剂量组,每组10只。空白组与模型组给予蒸馏水0.1g/(kg·d),塞来昔布胶囊组给予塞来昔布胶囊0.1g/(kg·d),小金丸高、中、低剂量组给予小金丸0.8、0.4、0.2g/(kg·d),以灌胃为给药形式,各级药物灌胃前皆配制为混悬液。各组大鼠灌胃均1次/d,连续给药4周后,断颈处死大鼠,无菌条件下摘除前列腺,10%中性甲醛液固定。免疫组化法并结合图形图像分析系统检测前列腺组织COX-2表达的变化。结果:模型组大鼠前列腺组织COX-2呈强表达,小金丸高、中剂量组及对照组较模型组COX-2表达明显降低(P<0.01),小金丸高剂量组COX-2表达较中、低剂量组降低(P<0.01)。结论:小金丸可抑制前列腺炎性疼痛大鼠前列腺组织COX-2的表达,并可能通过此抑制作用而达到缓解疼痛的目的。
OBJECTIVE: To observe the expression of cyclooxygenase-2 (COX-2) in prostatic tissue of prostatitis pain rats and to explore the possible mechanism of Xiaojin Pill in treatment of prostatitis pain. METHODS: Sixty male Wistar rats were randomized to receive 10 blank control groups. Intraperitoneal injection of sterile water for injection into the prostate was performed. The remaining 50 rats were injected with Freund’s complete adjuvant (CFA) to prepare prostatic inflammatory pain models. After that, they were randomly divided into model group, celecoxib capsule group, Xiaojin Pill high, middle, and low dose groups, with 10 in each group. The distilled water 0.1g/(kg·d) was given to the blank group and the model group, the celecoxib capsule was given 0.1g/(kg·d) by the celecoxib capsule group, and the Xiaojinwan 0.8, 0.4 was given by the high, middle, and low dose group of the Xiaojin Pill. , 0.2g/(kg·d), with gavage as the form of administration, all levels of drug were prepared as a suspension before gavage. Rats in each group were given intragastric administration once a day for 4 weeks. After continuous administration for 4 weeks, the rats were killed by cervical dislocation. The prostate was removed under aseptic conditions and 10% neutral formalin was fixed. The expression of COX-2 in prostate tissue was detected by immunohistochemistry combined with graphic image analysis system. Results: The expression of COX-2 in the prostate tissue of the model group was significantly higher than that of the model group (P<0.01). The expression of COX-2 was higher in the high-dose group of Xiaojin Pills than in the middle group. The low dose group decreased (P<0.01). Conclusion: Xiaojin Pill can inhibit the expression of COX-2 in prostatic tissue of rats with prostatitis pain, and may achieve the purpose of relieving pain through this inhibitory effect.