论文部分内容阅读
偏分离是指遗传标记的观察频率偏离预期的孟德尔式分离频率的现象,其可以增加群体中杂合等位基因或者异型染色体的频率,是一种重要的进化动力。本研究以粳稻材料日本晴及籼稻材料千粒稻为亲本获得80株F2群体,构建了一张含92对SSR标记和44对Indel标记的遗传图谱。结果发现,F2群体中,116个标记表现为偏分离现象(85.29%),其中79个极度偏分离(68.10%)。偏分离标记中由合子偏分离引起的有20个,配子偏分离引起的93个,RM3484,RM3702,os7-38.4既不是由合子选择引起也不是由配子选择引起。Indel标记偏分离率(97.72%)明显高于SSR标记偏分离率(79.34%),且所有偏分离标记皆偏向于千粒稻。结合本研究结果,对偏分离现象产生的原因进行了分析。
Partial segregation refers to the phenomenon that the observed frequency of genetic markers deviates from the expected Mendelian segregation frequency, which can increase the frequency of heterozygous alleles or heterozygous chromosomes in a population and is an important evolutionary motivation. In this study, 80 F2 populations were obtained from japonica rice japonica and indica rice grain, and a genetic map containing 92 pairs of SSR markers and 44 pairs of Indel markers was constructed. The results showed that in the F2 population, 116 markers showed partial segregation (85.29%), of which 79 were extremely segregated (68.10%). Partial segregation markers caused by zygotic partial separation of 20, gamete partial separation caused 93, RM3484, RM3702, os7-38.4 neither by the zygotic selection nor by gamete selection caused. The partial segregation rate of Indel was 97.72%, which was significantly higher than that of SSR (79.34%), and all partial segregation markers were biased toward one-thousand-grain rice. Combined with the results of this study, the causes of partial segregation were analyzed.