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目的探讨使用内镜下电烧灼的方法建立动物食管良性狭窄模型的可行性,为研究食管良性狭窄提供一个操作简单,烧伤范围和部位易控的动物模型。方法新西兰大白兔24只,随机分为空白对照组、实验Ⅰ组、Ⅱ组和Ⅲ组四组,分别予以仅行胃镜检查、内镜下10 W、30 W和50 W电凝功率烧灼食管中下段等处理,建立食管良性狭窄模型。观察各组实验动物造模前后体重变化、食管胃镜下改变、X线食管造影下食管内径、食管病理改变、狭窄指数和食管组织羟脯氨酸含量。结果空白对照组和实验Ⅰ组在实验后4周时的体重[(2.930±0.078)kg,(2.773±0.114)kg]均较实验前[(2.493±0.116)kg,(2.433±0.087)kg]明显增加(P均<0.01),食管内径[(11.200±0.632)mm,(11.100±0.874)mm较实验前[(11.350±0.789)mm,(11.233±0.742)mm]无明显变化(P均>0.05)。与实验前相比,实验Ⅱ组在实验后4周时的体重[(2.523±0.078)kg vs.(2.095±0.124)kg,P<0.01]和食管内径[(11.200±0.894)mm vs.(5.980±0.622)mm,P<0.01]则明显减小。实验后4周时实验Ⅰ组和实验Ⅱ组的组织病理学评分(1.333±0.516,3.000±0.707)和羟脯氨酸含量[(1.057±0.081)μg/mg,(1.400±0.113)μg/mg]均较空白对照组[0,(0.810±0.038)μg/mg]明显增加(P均<0.01)。在狭窄指数方面,实验后4周时实验Ⅰ组(0.223±0.040)与空白对照组(0.220±0.024)无统计学差异(P>0.05),而实验Ⅱ组则明显增高(0.484±0.117,P<0.01)。实验Ⅲ组造模后食管烧伤严重,动物均早期死亡。结论在内镜下采用电凝功率30 W烧灼食管可成功建立食管良性狭窄模型,该方法操作简单、烧伤程度和部位易控。
Objective To explore the feasibility of using endoscopic electrosurgery to establish a model of benign esophageal stenosis in rats and to provide an animal model with simple operation, easy-to-burn range and easy-to-control site for the study of esophageal stricture. Methods Twenty-four New Zealand white rabbits were randomly divided into blank control group, experimental group Ⅰ, group Ⅱ and group Ⅲ, respectively. Gastroscopy was performed only. Endoscopic electrocoagulation power 10 W, 30 W and 50 W The next paragraph and other treatment, the establishment of esophageal stricture model. The changes of body weight, esophageal endoscopy, esophageal diameter, esophageal pathological changes, stenosis index and hydroxyproline content in esophageal tissue were observed before and after modeling. Results The body weight of the blank control group and the experimental group Ⅰ at the 4th week after the experiment [(2.930 ± 0.078) kg and (2.773 ± 0.114) kg] were significantly higher than those before the experiment [(2.493 ± 0.116) kg and (2.433 ± 0.087) kg] (11.11 ± 0.632) mm and (11.100 ± 0.874) mm, respectively, compared with those before the experiment [(11.350 ± 0.789) mm, (11.233 ± 0.742) mm] (P <0.01) 0.05). Compared with that before experiment, the body weight in group Ⅱ was significantly higher than that in group Ⅱ [(2.523 ± 0.078) kg vs. (2.095 ± 0.124) kg, P <0.01] and esophageal diameter [(11.200 ± 0.894) mm vs. 5.980 ± 0.622) mm, P <0.01] was significantly reduced. The histopathological score (1.333 ± 0.516, 3.000 ± 0.707) and hydroxyproline content [(1.057 ± 0.081) μg / mg, (1.400 ± 0.113) μg / mg ] Were significantly higher than those in the blank control group [0, (0.810 ± 0.038) μg / mg] (all P <0.01). In terms of stenosis index, there was no significant difference between experimental group Ⅰ (0.223 ± 0.040) and blank control group (0.220 ± 0.024) at 4 weeks after the experiment (P> 0.05), and in experimental group Ⅱ (0.484 ± 0.117, P <0.01). In the third experiment group, esophageal burns were severe after modeling, and the animals died early. Conclusion The model of benign esophageal stenosis can be successfully established by endoscopic electrocoagulation with a power of 30 W. The method is simple in operation, easy to burn and easy to control.