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以野生和栽培甜瓜为试材,通过人工接种白粉病菌,对两份材料的抗性进行了鉴定,结果表明野生种质对甜瓜白粉病菌生理小种Race1具有抗性。基因测序发现野生种质与栽培品种的MLO基因序列相差85个碱基。进一步利用荧光标记的农杆菌介导法将Cm MLO2基因在野生种质中表达,白粉病菌能够感染阳性转化植株,说明Cm MLO2突变使野生种质缺失MLO蛋白,导致对白粉病的负调控作用被解除,从而启动了对白粉病菌的广谱抗性。
The wild and cultivated muskmelon were used as materials, and the resistance of the two materials was identified through artificial inoculation of powdery mildew germplasm. The results showed that the wild germplasm was resistant to race1 of Race 1 of powdery mildew of Melon. Gene sequencing found that the wild germplasm and cultivars MLO gene sequence difference of 85 bases. Further, Cm MLO2 gene was expressed in wild germplasms by fluorescent-labeled Agrobacterium-mediated method. Powdery mildew was able to infect the positive transgenic plants, indicating that Cm MLO2 mutation caused MLO deficiency in wild germplasm resulting in the negative regulation of powdery mildew Lifted, thus starting a broad spectrum resistance to powdery mildew.