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本文采用高效液相色谱法,以甲醇+水=65+35为流动相,采用 ODS-C18色谱柱在同一色谱条件下,各元素的标准偏差小于0.3%,标准加入回收率在98.6%~99.3%之间,实现各有效成分的理想分离和定量测定。
The standard deviation of each element was less than 0.3% by ODS-C18 column under the same chromatographic conditions using high performance liquid chromatography with methanol + water = 65 +35 as the mobile phase. The standard recoveries ranged from 98.6% to 99.3 %, To achieve the ideal separation of the active ingredient and quantitative determination.