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研究了顺氨氯铂(CDDP)、丝裂霉素C和阿霉素对巨噬细胞产生肿瘤坏死因子(TNF)的影响,与不刺激氧自由基(ROS)释放的5氟脲嘧啶作对照。取C57B1/6雌鼠的巨噬细胞,洗3次后悬浮于不含酚红的DMEM、10％小牛血清、1％谷酰胺、2％青-链霉素的培养基中。将100μ1(2×10~细胞/ml)分注于平底组织培养皿内侧60井内,在95％空气/5％CO_2和37℃下孵育2小时,再用100mm~3Hank平衡盐溶液洗去不粘附的细胞,再在培养基中孵育一夜。残留的粘附细胞90％是巨噬细胞。将巨噬细胞与
The effects of cisplatin (CDDP), mitomycin C, and doxorubicin on tumor necrosis factor (TNF) production by macrophages were studied, compared with 5-fluorouracil that did not stimulate the release of oxygen free radicals (ROS). . Macrophages from C57B1/6 female mice were washed three times and suspended in a medium containing no phenol red DMEM, 10% calf serum, 1% glutamine, and 2% cyanine-streptomycin. Dispense 100 μl (2 x 10 cells/ml) into the well 60 inside the flat-bottomed tissue culture dish and incubate for 2 hours at 95% air/5% CO 2 and 37°C, and then wash off with 100mm~3 Hank balanced salt solution. Attach the cells and incubate overnight in culture medium. 90% of the remaining adherent cells are macrophages. The macrophage with