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番茄不孕病毒是福建省切花菊病毒病的主要病原病毒，平均带毒率高达８５．９％。为探讨其脱毒技术及其可行性，以福建省种植的菊花品种为试验材料，采用①热处理（白天４０℃、夜间３０℃，５～２０ｄ），②热处理结合茎尖培养，③热处理结合茎尖培养再二次培养等３种处理，经生物学鉴定、电镜观察和间接酶联免疫吸附试验检测（ＩＤ－ＥＬＩＳＡ），３种处理的脱毒率分别为１３．３％～２０．０％、４１．６％～６０．０％和８３．３％～９０．０％。此外，还进行了不同品种和外植体长度对切花菊组织培养的诱导率及脱毒率的影响试验。结果表明，热处理（白天４０℃、夜间３０℃，１０～２０ｄ）结合茎尖培养（取茎尖０．５～１．０ｍｍ）再二次培养是脱除切花菊的番茄不孕病毒的最有效方法，可在大田生产中应用推广。
Infectious tomato virus is the major pathogenic virus of cut flower chrysanthemum in Fujian Province, with an average rate of up to 85.9%. In order to explore its detoxification technology and its feasibility, the varieties of chrysanthemum grown in Fujian Province were used as experimental materials, ① heat treatment (40 ℃ during the day, 30 ℃ at night, 5 ~ 20d), ② heat treatment combined with shoot culture, ③ heat treatment combined with stem The results showed that the detoxification rates of the three treatments were 13.3% ~ 20.0% by ID-ELISA, biological identification, electron microscope observation and indirect enzyme-linked immunosorbent assay (ID-ELISA) , 41.6% -60.0% and 83.3% -90.0%. In addition, the effects of different varieties and explant lengths on the induction rate and detoxification rate of cut chrysanthemum tissue culture were also tested. The results showed that heat treatment (daytime 40 ℃, night 30 ℃, 10 ~ 20d) combined with shoot tip culture (take 0.5 ~ 1.0mm shoot tip) re-culture is the removal of cut chrysanthemum tomato infertility virus most effective Method can be applied in the field of production promotion.