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目的检测和评价染料木黄酮的诱变性,为其安全应用提供毒理学依据。方法采用小鼠淋巴瘤细胞实验(微孔板法)评价染料木黄酮的诱变性,诱变性检测采用3h和24h染毒两种处理方式观察受试物对L5178Y细胞的诱变性。结果在染料木黄酮诱变性检测中,3h染毒时,随着剂量增加,tk位点总突变频率升高,5、10和20μg/mL剂量组tk位点总突变频率与溶剂对照组比较差异有统计学意义(P<0.01);24h染毒时,随着剂量增加,tk位点总突变频率升高,与溶剂对照组比较,5、10μg/mL剂量组tk位点总突变频率高于溶剂对照组,差异有统计学意义(P<0.01);3h染毒和24h染毒相同剂量组间比较,tk位点总突变频率差异无统计学意义(P>0.05)。结论在此实验条件下,染料木黄酮(5~20)μg/mL能诱导L5178Y细胞TK基因突变,并呈良好的剂量反应关系,但3h和24h处理的突变频率差异无统计学意义(P>0.05)。
Objective To detect and evaluate the mutagenicity of genistein and provide a toxicology basis for its safety application. Methods The mouse lymphoma cell assay (microplate method) was used to evaluate the mutagenicity of genistein. The mutagenicity of the genistein was assayed by 3 h and 24 h treatment. The mutagenicity of the genistein on L5178Y cells was observed. Results Genistein mutagenicity test, 3h exposure, as the dose increased, total tk mutation frequency increased, 5,10 and 20μg / mL dose group total tk mutation frequency compared with the solvent control group The difference was statistically significant (P <0.01). At 24h, the total mutation frequency of tk locus increased with dose increasing. Compared with the solvent control group, the total mutation frequency of tk locus in 5,10μg / mL dose group was high (P <0.01). There was no significant difference in the total mutation frequency at tk locus between 3 h and 24 h groups (P> 0.05). Conclusions Genistein (5 ~ 20) μg / mL can induce the TK gene mutation in L5178Y cells with good dose response under the experimental conditions. However, there was no significant difference in the frequency of mutation between 3h and 24h treatment (P> 0.05).