采用生殖生物学方法研究了转bar基因水稻品种Y0 0 0 3和 99 t(父本 )与无芒稗 (母本 )的基因漂移。通过光学显微镜观察稗草与转bar基因水稻手工杂交后 30min、1～ 4h水稻花粉在稗草柱头上的萌发、生长情况 ,并与稗草开花后相应时间的花粉萌发、生长情况相比较。结果表明 ,2个水稻品种的花粉在稗草柱头上的萌发生长情况相似 ,且与稗草自花授粉相应时间的花粉萌发、生长情况有极明显差异。稗草自花授粉后花粉粒萌发生长正常 ,在 30min时 85 %的花粉粒花粉管穿过柱头 ,正在凝缩和释放内容物或内容物已经释放的花粉粒百分率逐渐增加 ;杂交后转基因水稻花粉在稗草柱头上都不能正常萌发生长并未能穿过稗草柱头。从而判定稗草与转bar基因水稻的不亲和性 ,表现在水稻花粉不能在稗草柱头上正常萌发生长 ,更不能穿过稗草柱头。同时通过去雄稗草授转基因水稻花粉后不结籽也证明了两者的不亲和性 The gene transfer of bar transgenic rice Y0 0 0 3 and 99 t (male) and barnyardgrass (female) was studied by using reproductive biology method. The germination and growth of rice pollen on the barnyardgrass stigma were observed 30min and 1 ~ 4h after the hybridization between barnyardgrass and bar transgenic rice by hand under light microscope, and compared with the pollen germination and growth at the corresponding time after barnyard grass blooming. The results showed that the germination and growth of pollen of barnyardgrass in two rice cultivars were similar, and the pollen germination and growth of barnyardgrass were significantly different at the corresponding time. After pollination, the pollen grains germinated and grew normally. At 30 minutes, 85% of the pollen grains passed through the stigma, and the percentages of pollen grains that were being condensed and released or the contents had been released gradually increased. After hybridization, the transgenic rice pollens Barnyardgrass can not germinate normally on the head and failed to cross barnyardgrass stigma. In order to determine the barnyardgrass transgenic bar transgenic rice incompatibility, the performance of rice pollen can not germinate barnyardgrass germination and growth, but can not pass barnyardgrass stigma. At the same time, the non-seedlessness of transgenic rice