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目的： 以筛选恶性疟原虫ＦＣＣ１／ＨＮ 株λｇｔ１１ ｃＤＮＡ 表达文库所获得的强阳性克隆作基础， 对上述强阳性克隆的ｃＤＮＡ 插入片段进行ＤＮＡ 序列测定， 阐明相对应的新表达序列标签 （ＥＳＴｓ）， 作为发现新抗原基因的线索。方法：以ｃＤＮＡ 表达文库接头的较长链作ＰＣＲ引物、扩增ｃＤＮＡ 插入片段，将扩增产物克隆入Ｍ１３ ｍ ｐ１８测序载体， 进行部分ＤＮＡ 序列测定、编辑， 将之在ＧｅｎＢａｎｋ 中进行ＤＮＡ 序列同源性搜索比较和分析。结果： 获得１ 个Ｃ０３ 序列为已知恶性疟原虫热休克蛋白７０－２ 基因片段， 发现５ 个新的具有抗原意义的恶性疟原虫表达序列标记位 （ＥＳＴｓ）。结论： 这５ 个新的恶性疟原虫表达序列标记位为发现新的恶性疟原虫抗原基因奠定了基础。
OBJECTIVE: To detect the strong positive clones obtained from the λgt11 cDNA expression library of Plasmodium falciparum FCC1 / HN strain, the DNA sequences of the cDNA fragments of the strongly positive clones were determined and the corresponding new expressed sequence tags (ESTs) As a clue to discover new antigen genes. Methods: The longer strand of the cDNA expression library was used as a PCR primer to amplify the cDNA insert. The amplified product was cloned into the M13 m p18 sequencing vector for partial DNA sequencing and editing. The DNA sequence was identical Source Search Comparison and Analysis. Results: One C03 sequence was obtained from the known 70-2 gene fragment of heat shock protein of Plasmodium falciparum. Five novel antigenic sequences of Plasmodium falciparum expressed sequence tags (ESTs) were found. Conclusion: The five new markers of Plasmodium falciparum expression sequence laid the foundation for the discovery of new Plasmodium falciparum antigen genes.