目的：了解新生儿在感染状态下血清粒细胞集落刺激因子（ＧＣＳＦ）的变化。方法：采用酶标双抗体夹心法（ＥＬＩＳＡ法）测定５０例感染新生儿和２０例非感染新生儿血清ＧＣＳＦ水平。结果：感染组新生儿血清ＧＣＳＦ阳性率达７４％，其血清浓度和ＯＤ值均显著高于非感染组新生儿（Ｐ＜００１）。结论：新生儿在感染状态下血清ＧＣＳＦ明显升高，测定体液中ＧＣＳＦ水平对于新生儿在感染性疾病的诊断、鉴别诊断以及指导临床合理选用抗生素具有重要的参考价值 Objective: To understand the changes of serum granulocyte colony stimulating factor (GCSF) in neonates during infection. Methods: Enzyme-linked double antibody sandwich method (ELISA method) was used to determine serum G-CSF levels in 50 infants with neonatal infection and 20 non-infected neonates. Results: The positive rate of serum G-CSF in neonates was 74% in infected group, and the serum concentration and OD value in neonates were significantly higher than those in non-infected neonates (P <001). Conclusion: Neonates serum G-CSF significantly increased in infected state, determination of G-CSF levels in the body fluids for neonatal diagnosis of infectious diseases, differential diagnosis and clinical rational use of antibiotics has important reference value