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目的:观察参竹心康汤对慢性心力衰竭(CHF)大鼠心肌纤维化以及心肌基质金属蛋白酶-9、基质金属蛋白酶抑制剂-1的影响,探讨该方抑制心肌纤维化,改善心室重构的可能作用机制。方法:从70只SD大鼠中随机取14只为假手术组,其余大鼠均为手术组,手术组大鼠采用腹主动脉缩窄法复制慢性心力衰竭大鼠模型,再将手术组大鼠按随机排列表法随机分为模型组7只、卡托普利组8只、参竹心康汤小剂量组7只、中剂量组8只、大剂量组8只,假手术组及模型组均予蒸馏水(10 m L/kg)灌胃,卡托普利组予卡托普利混悬液(7.8 mg/kg)灌胃,参竹心康汤小、中、大剂量组分别予参竹心康汤中药煎液(含生药4.5 g/m L)11 g/kg、22 g/kg、44 g/kg灌胃,1次/d,连续给药8周。用Masson染色后图像分析测量心肌胶原容积分数(CVF),ELISA法检测心肌基质金属蛋白酶-9(MMP-9)、基质金属蛋白酶抑制剂-1(TIMP-1)水平。结果:与模型组比较,参竹心康汤小、中、大剂量组大鼠心肌CVF和MMP-9水平明显下降,TIMP-1水平升高,差异均有统计学意义(P<0.01),且在一定剂量范围内,随着剂量的增加,心肌MMP-9水平及CVF逐渐下降,TIMP-1水平逐渐升高。结论:参竹心康汤有抗CHF心肌纤维化的作用,其机制可能与抑制MMP-9表达,促进TIMP-1表达有关。
Objective: To observe the effect of shenzhu xinkang decoction on myocardial fibrosis and myocardial matrix metalloproteinase-9 and matrix metalloproteinase-1 in chronic heart failure (CHF) rats, and to explore the effect of this recipe on myocardial fibrosis and ventricular remodeling The possible mechanism of action. Methods: Fourteen of 70 SD rats were randomly divided into sham operation group and the other rats were operated. The rats in operation group were treated by abdominal aorta constriction to copy the model of chronic heart failure. The rats were randomly divided into seven groups according to the randomized table method: captopril group (n = 8), Shenzhuoxinkang low dose group (n = 7), middle dose group (n = 8), high dose group (n = 8), sham operation group and model Groups were given distilled water (10 m L / kg) by gavage, captopril group captopril suspension (7.8 mg / kg) by gavage, Shenzhuoxinkang small, medium and large dose groups were given Shenzhuoxinkang Decoction (containing 4.5 g / m L crude drug) 11 g / kg, 22 g / kg, 44 g / kg gavage once daily for 8 weeks. Myocardial collagen volume fraction (CVF) was measured by Masson staining, myocardial MMP-9 and TIMP-1 were detected by ELISA. Results: Compared with the model group, the myocardial CVF and MMP-9 levels were significantly decreased and the TIMP-1 levels were increased in the small, middle and large dose groups of Shenzhuxinkang decoction group (P <0.01) And in a certain dose range, with the increase of dose, myocardial MMP-9 level and CVF gradually decreased, and TIMP-1 level gradually increased. Conclusion: Shenzhu xinkang decoction has the effect of anti-CHF myocardial fibrosis, the mechanism may be related to inhibiting the expression of MMP-9 and promoting the expression of TIMP-1.