论文部分内容阅读
采用SDS-聚丙烯酰胺凝胶电泳技术(SDS-PAGE)和PAGE电泳技术分别对安徽不同地区的46个辣椒疫霉进行可溶性蛋白和酯酶同工酶分析,从蛋白质和酶学水平上分析辣椒疫霉的生理生化特征。可溶性蛋白电泳表明,每个辣椒疫霉分离出了25~37条谱带,平均31.5条。其中具有多态性的条带共有24条,多态性比率达64.9%,可溶性蛋白图谱与地理来源相关,但与致病力不相关。酯酶同工酶PAGE电泳显示辣椒疫霉菌株各有1~8条条带,Rf值为0.05~1.00,不同辣椒疫霉菌株间某些同工酶谱带数和同一迁移率谱带的颜色和宽度差异显著,其中弱致病力菌株的条带数为1~3条,说明菌株的致病力差异可在酯酶同工酶水平上得到反映。
SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and PAGE electrophoresis were used to analyze the protein and esterase isozyme of 46 P. capsici isolated in different regions of Anhui Province. Pepper Physiological and biochemical characteristics of Phytophthora capsici. Soluble protein electrophoresis showed that 25 ~ 37 bands were isolated from each Phytophthora capsici with an average of 31.5. There were 24 bands with polymorphism, the rate of polymorphism was 64.9%. The map of soluble protein was related with geographical origin, but not with pathogenicity. Esterase isoenzyme PAGE electrophoresis showed that there were 1 ~ 8 bands in Phytophthora capsici and Rf values of 0.05 ~ 1.00. The isozyme bands and the same mobility bands of different Phytophthora capsici strains were different And the width was significantly different. The number of bands of weak virulence strains was 1 ~ 3, indicating that the differences in pathogenicity of the strains could be reflected in esterase isozyme level.