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利用ＲＡＰＤ分子标记技术，结合已知系谱信息，对国内外不同来源的２５个抗（感）黄萎病的棉花品种（系）进行特征及特性分析。从被测试的４０个引物中选取对２５个棉花品种（系）ＤＮＡ扩增表现多态性的２６个随机引物，构建２５个棉花品种（系）ＤＮＡ指纹图谱，并进一步分析了遗传多样性。结果表明：供试的２５个棉花品种（系）可划分为４个类群，这与其系谱来源及抗黄萎病的抗源来源基本吻合。第Ⅰ类为国外引入的抗黄萎病品种；第Ⅱ类为陕棉、辽棉系统；第Ⅲ类为遗传基础复杂，从病圃定向选择培育的抗黄萎病品种（系）；第Ⅳ类为长江流域感黄萎病品种“太仓１２１”。该研究从ＤＮＡ水平上揭示了中国现有的抗（耐）黄萎病品种（系）的遗传真实性。
The characteristics and characteristics of 25 resistant (susceptible) Verticillium wilt cotton varieties (lines) from different sources at home and abroad were analyzed by using RAPD molecular markers and known pedigree information. A total of 26 random primers were selected from 40 primers to amplify the polymorphic DNA of 25 cotton cultivars. DNA fingerprinting of 25 cotton cultivars (lines) was constructed and the genetic diversity was further analyzed. The results showed that the 25 tested cotton cultivars (lines) could be divided into four groups, which were basically consistent with their pedigree source and the source of resistance to Verticillium wilt. The first category is the introduction of foreign varieties of resistance to Verticillium wilt; the second category is the Shaanxi cotton, the Liaodian system; the third category is the genetic basis of complex selection from the nursery to cultivate the resistance to Verticillium wilt varieties (lines); Ⅳ The category is the “Taicang 121” in the Yangtze River valley. This study revealed the genetic authenticity of the existing resistant (resistant) Verticillium wilt varieties (lines) at the DNA level in China.