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目的采用大鼠进行不同建模,并比较研究全面,寻找最佳的脂肪肝造模方法。方法 Wistar大鼠随机分四组:阴性对照组饲以普通饲料;模型A、B、C组饲以高脂饲料,A、B组分别每日以50%、40%乙醇灌胃;C组后肢皮下注射四氯化碳。实验7周,以断髓法处死大鼠,观察三种脂肪肝模型的肝脏病理学改变并进行比较分析。结果 A、B、C三组大鼠肝指数无明显差异,A组大鼠肝脏出现弥漫性中度脂肪变性,汇管区可见炎性细胞浸润;B组大鼠肝脏出现轻度脂肪变性,胞浆中可见大小不等的脂肪空泡;C组大鼠肝脏出现轻度脂肪变性,肝小叶可见点灶状肝细胞坏死,淋巴细胞浸润。结论 C组的造模方法简便易行,模型复制率高,为制备动物脂肪肝模型的首选。
OBJECTIVE: To adopt different modeling methods in rats and compare them comprehensively to find out the best method of making fatty liver. Methods Wistar rats were randomly divided into four groups: negative control group fed with normal diet; model A, B, C group fed with high-fat diet, A, B group were daily 50%, 40% Subcutaneous injection of carbon tetrachloride. Seven weeks after the experiment, the rats were killed by the method of broken pulp, and the liver pathological changes of the three fatty liver models were observed and compared. Results There was no significant difference in liver index between A, B and C groups. A group of rats showed diffuse moderate fatty degeneration in liver and inflammatory cell infiltration in portal area. In group B, mild fatty degeneration was found in liver and cytoplasm In the group C, mild fatty degeneration was observed in the liver of the rats, necrosis of focal hepatocytes and infiltration of lymphocytes in the hepatic lobules. Conclusion The methods of modeling in group C are simple and easy to operate, and the model replication rate is high, which is the first choice for preparing animal model of fatty liver.