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用原位缺口翻译法（ＩＮＴ）比较了细胞凋亡和坏死所伴随的ＤＮＡ断裂（ＤＳＢ）类型。ＩＮＴ实验条件为：取大鼠小肠制备冰冻切片，乙醇／醋酸固定，加２０μｍｏｌ／Ｌｂｉｏｔｉｎ－１１－ｄＵＴＰ、０．２ＩＵ／ＬＤＮＡ聚合酶Ⅰ，于３７℃中孵育３ｈ。正常大鼠小肠绒毛顶端上皮细胞可迅速被染色；而位于肠绒毛基底部的Ｓ期上皮细胞则不着色，仅在用蛋白酶Ｋ处理后才着色。细胞凋亡标本取自皮下注射氢化可的松（１０ｍｇ／１００ｇ体重）的大鼠胸腺；细胞坏死用ＣＣｌ４（１００μｌ／１００ｇ体重）处理的大鼠肝脏。结果表明，经激素处理的胸腺皮质细胞核只有在蛋白酶Ｋ处理后才被染色，而ＣＣｌ４处理后的肝细胞核不必用蛋白酶Ｋ消化就能直接显色。说明细胞凋亡的ＤＳＢ显色特性不同于细胞坏死。
DNA fragmentation (DSB) types associated with apoptosis and necrosis were compared using in situ nick translation (INT). The experimental conditions of INT were as follows: Frozen sections were prepared from the small intestine of rats and fixed in ethanol / acetic acid. After adding 20μmol / L biotin-11-dUTP and 0.2IU / LDNA polymerase I, the cells were incubated at 37 ℃ for 3h. Normal rat epithelial cells of the villus of the small intestine can be quickly stained; while the S-phase epithelial cells located at the base of the villi are not stained and only stained with proteinase K treatment. Apoptotic specimens were obtained from rat thymus subcutaneously injected with hydrocortisone (10 mg / 100 g body weight); necrosis of rat livers treated with CCl4 (100 μl / 100 g body weight). The results showed that the hormonally-treated thymus nucleus was stained only after treatment with proteinase K, whereas the CCl4 -treated hepatocyte nucleus did not have to be digested directly with proteinase K to develop a direct color. The DSB color development characteristic of apoptosis is different from that of cell necrosis.